calculate the [PNP] in μM for each of these samples. For Table 2: Calculate the volumes of the 100 mM PNPP stock solution and buffer (final volume of 1.00 ml) needed to get each of the substrate concentrations.
calculate the [PNP] in μM for each of these samples. For Table 2: Calculate the volumes of the 100 mM PNPP stock solution and buffer (final volume of 1.00 ml) needed to get each of the substrate concentrations.
Basic Clinical Lab Competencies for Respiratory Care: An Integrated Approach
5th Edition
ISBN:9781285244662
Author:White
Publisher:White
Chapter14: Introduction To Respiratory Care Pharmacology
Section: Chapter Questions
Problem 5SEPT
Related questions
Question
For Table 1:
calculate the [PNP] in μM for each of these samples.
For Table 2:
Calculate the volumes of the 100 mM PNPP stock solution and buffer (final
volume of 1.00 ml) needed to get each of the substrate concentrations.
![(WATCH YOUR UNITS OF MEASUREMENT)
Buffer
(μl)
[PNP] in uM
1500
1490
1480
1470
1460
5
1450
6
1440
7
75
1425
8
100
1400
Note that in the PNP column, uM = μmoles/liter.
Table 1
Tube #
Blank
1
2
3
+10
4
PNP (μl)
0.5 mM stock
0
10
20
30
40
50
60
Trial 1
A405
Trial 2
A405](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F51044012-1e7d-4f53-9311-c2b96c730020%2F5d6df19a-c6fe-4ff0-94ab-39efc93091f0%2Fckg6w7sr_processed.jpeg&w=3840&q=75)
Transcribed Image Text:(WATCH YOUR UNITS OF MEASUREMENT)
Buffer
(μl)
[PNP] in uM
1500
1490
1480
1470
1460
5
1450
6
1440
7
75
1425
8
100
1400
Note that in the PNP column, uM = μmoles/liter.
Table 1
Tube #
Blank
1
2
3
+10
4
PNP (μl)
0.5 mM stock
0
10
20
30
40
50
60
Trial 1
A405
Trial 2
A405
![Table 2
Tube #
Blank
1
2
3
4
5
PNPP (ul)
100 mM stock
0
Buffer (μl)
1000
Enzyme
(μl)
0
5
5
5
5
5
Final volume
(μl)
1000
1000
1000
1000
1000
1000
[PNPP] in uM
0
200
400
600
800
1000](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F51044012-1e7d-4f53-9311-c2b96c730020%2F5d6df19a-c6fe-4ff0-94ab-39efc93091f0%2Fj9zsw2u_processed.jpeg&w=3840&q=75)
Transcribed Image Text:Table 2
Tube #
Blank
1
2
3
4
5
PNPP (ul)
100 mM stock
0
Buffer (μl)
1000
Enzyme
(μl)
0
5
5
5
5
5
Final volume
(μl)
1000
1000
1000
1000
1000
1000
[PNPP] in uM
0
200
400
600
800
1000
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