Calculate the total number of bacteria in your yogurt container three times (in duplicate). Begin by choosing a dilution plate that has 30-300 colonies on its surface. This will be your #CFU's/#ml #CFU's x Dil Factor x Tube 1 total vol (ml) x yogurt container mass (g) = total # of bacteria in yogurt (g) in Tube 1 yogurt container #ml 1 4. Since you have two plates with the same dilution (duplicates), calculate the total number of bacteria in your yogurt two times. Circle your three answers. Make sure to include the correct values with your numbers. 5. Average your two answers above for a more accurate representation of the total number of bacteria in your yogurt container.

Aquaculture Science
3rd Edition
ISBN:9781133558347
Author:Parker
Publisher:Parker
Chapter2: Aquatic Plants And Animals
Section: Chapter Questions
Problem 2KA
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Total amount of yogurt in the container: 170gram

The actual amount of yogurt added to Tube 1 is 1 gram.

The actual volume in Tube 1 is 10ml. 

Please help with number 4 and 5. Thanks! 

 

Calculate the total number of bacteria in your yogurt container three times (in duplicate).
Begin by choosing a dilution plate that has 30-300 colonies on its surface. This will be your #CFU's/#ml
# CFU's x Dil" Factor x Tube 1 total vol (ml) x yogurt container mass (g) = total # of bacteria in
yogurt (g) in Tube 1
yogurt container
#ml
1
4. Since you have two plates with the same dilution (duplicates), calculate the total number of bacteria in your
yogurt two times. Circle your three answers. Make sure to include the correct values with your numbers.
5.
Average your two answers above for a more accurate representation of the total number of bacteria in your
yogurt container.
Transcribed Image Text:Calculate the total number of bacteria in your yogurt container three times (in duplicate). Begin by choosing a dilution plate that has 30-300 colonies on its surface. This will be your #CFU's/#ml # CFU's x Dil" Factor x Tube 1 total vol (ml) x yogurt container mass (g) = total # of bacteria in yogurt (g) in Tube 1 yogurt container #ml 1 4. Since you have two plates with the same dilution (duplicates), calculate the total number of bacteria in your yogurt two times. Circle your three answers. Make sure to include the correct values with your numbers. 5. Average your two answers above for a more accurate representation of the total number of bacteria in your yogurt container.
+
3.
CFU's
Tube #
Tube Dilution
Dilution Factor
(undil)
Plate 1
TMTC
TMTC
1
1/10____
1/10
03-A
--
>
Fill in the Tube dilution and Dilution Factor for each tube:
LAIN
Jaim
017
0.1ml
0.1 ml
4
5
O O O O O O O
DOO
Plate 2
TMTC
TMTC
2
M
1/10
V
1.1.
0.1 ml
3
Plate 3
TMTC
TMTC
1/10
Þ
4
Plate 4
TMTC
TMTC
1/10
5
1/10
Þ
1/100 1/1000 1/10000_ 1/100000
Plate 5
TMTC
TMTC
-
6
10
1/10
laim
0.1 ml
Plate 6
34
57
7
1/10_
gel
1/1000000_1/0000000
V
Plate 7
9
Transcribed Image Text:+ 3. CFU's Tube # Tube Dilution Dilution Factor (undil) Plate 1 TMTC TMTC 1 1/10____ 1/10 03-A -- > Fill in the Tube dilution and Dilution Factor for each tube: LAIN Jaim 017 0.1ml 0.1 ml 4 5 O O O O O O O DOO Plate 2 TMTC TMTC 2 M 1/10 V 1.1. 0.1 ml 3 Plate 3 TMTC TMTC 1/10 Þ 4 Plate 4 TMTC TMTC 1/10 5 1/10 Þ 1/100 1/1000 1/10000_ 1/100000 Plate 5 TMTC TMTC - 6 10 1/10 laim 0.1 ml Plate 6 34 57 7 1/10_ gel 1/1000000_1/0000000 V Plate 7 9
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