Cell type B (transport protein mutant) Time IN [Na+] 150mM 0 10 ms 20 ms 40mM 40mM OUT [Na+] 15mM 125mM 125mM IN [ATP] 200mM 70mM 70mM IN [Glu] 5mM 5mM 5mM OUT [Glu] 200mM 200mM 200mM Q4. Now, analyze a similar data set for cell type B above. Add an arrow labelled with 1° to indicated where primary active transport occurred. Add an arrow labelled with 2° to indicate where secondary active transport occurred (if it occurs). Q5. What is not occurring in Cell B (mutant) compared to Cell A (non mutant) ? Therefore, what do you conclude about the normal role of this transport protein?

Cell type B (transport protein mutant) Time IN [Na+] 150mM 0 10 ms 20 ms 40mM 40mM OUT [Na+] 15mM 125mM 125mM IN [ATP] 200mM 70mM 70mM IN [Glu] 5mM 5mM 5mM OUT [Glu] 200mM 200mM 200mM Q4. Now, analyze a similar data set for cell type B above. Add an arrow labelled with 1° to indicated where primary active transport occurred. Add an arrow labelled with 2° to indicate where secondary active transport occurred (if it occurs). Q5. What is not occurring in Cell B (mutant) compared to Cell A (non mutant) ? Therefore, what do you conclude about the normal role of this transport protein?

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter9: Cell Communication

Section: Chapter Questions

Problem 1ITD

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Vesicle transport occurs between the ER and the Golgi in bothdirections. Let’s suppose a researcher added a drug to cells thatinhibited vesicle transport from the Golgi to the ER but did not affectvesicle transport from the ER to the Golgi. If you observed cellsmicroscopically after the drug was added, what would you expect to seehappen over the course of 1 hour?a. The ER would get smaller, and the Golgi would get larger.b. The ER would get larger, and the Golgi would get smaller.c. The ER and Golgi would stay the same size.d. Both the ER and Golgi would get larger.e. Both the ER and Golgi would get smaller
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Calculate ΔGinward. Is energy required for transport to happen? The internal pH of a lysosome= 4.0 and the pH of the cytosol is 7.0. For a cell at 25°C with a -40 mV lysosome membrane potential. What is the ΔGinward for the proton gradient into the lysosome?
Suppose calcium ion is maintained within an organelle at a concentration1000 times greater than outside the organelle (T = 37 °C). Assuming the membrane is permeable to Ca2+, what is the contribution of Ca2+ to the membrane potential? Which side of the organelle membrane is positive, and which is negative?
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Suppose calcium ion is maintained within an organelle at a concentration 1000 times greater than outside the organelle (T = 37 °C). Assuming the membrane is permeable to Ca2+, what is the contribution of Ca2+ to the membrane potential? Which side of the organelle membrane is positive, and which is negative?
If cells are treated with colchicine, a drug that promotes microtubule disassembly, the location of the ER and Golgi both change dramatically. Rather than being spread out quite widely through the cytoplasm, the ER appears to collapse into the area near the nucleus. (Recall that the ER membrane and the outer nuclear envelope are continuous with one another). In contrast, the Golgi stacks disperse from their usually more compact, central location to areas all over the cell. However, if colchicine is removed, and microtubules are allowed to reassemble, both organelles eventually return to their normal distribution in the cells. How do you account for the change in distribution of the ER & Golgi when microtubules are depolymerized? What protein(s), other than tubulins, allow normal cellular organization to be restored once colchicine is removed? (answer should include information to explain both the ER & Golgi)