Choices: Origin of replication Bubble SSBP RPA Sliding Clamp PCNA DNA Pol III Pol ε Pol δ DNA Pol I RNAse H Flap 1 DNA gyrase Pol α DNA helicase Primase Single chromosome Multiple points in chromosomes DNA ligase Not applicable 1. Origin of replication in eukaryotes 2. Joins the Okazaki fragments in leading strand 3. Dissociates after adding the few initial nucleotides in eukaryotes 4. Adds more nucleotides in the lagging strand of prokaryotes 5. Origin of replication in prokaryotes 6. Lessens the tension of supercoiling
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- Choices: Origin of replication Bubble SSBP RPA Sliding Clamp PCNA DNA Pol III Pol ε Pol δ DNA Pol I RNAse H Flap 1 DNA gyrase Pol α DNA helicase Primase Single chromosome Multiple points in chromosomes DNA ligase Not applicable 17. Start of replication in prokaryotes 18. Add nuclecleotides to the site of the removed prokaryotic primer 19. Dissociates after adding the few initial nucleotides in prokaryotes 20. Proof reading in prokaryotic DNA material 21. Start of replication in eukaryotes 22. Breaks the H-bonds of basesPLEASE HELP ME WITH THIS ONEMatch the statement to the corresponding agent/key player in DNA replication. Some items require more than one answer. Choices: Origin of replication Bubble SSBP RPA Sliding Clamp PCNA DNA Pol III Pol ε Pol δ DNA Pol I RNAse H Flap 1 DNA gyrase Pol α DNA helicase Primase Single chromosome Multiple points in chromosomes DNA ligase Not applicable Holds the processive enzyme in prokaryotes Start of replication in prokaryotes Holds the processive enzyme in eukaryotes Primosome in prokaryotes Dissociates after adding the few initial nucleotides in eukaryotes Adds more nucleotides in the lagging strand of prokaryotes Make primers for the replicative enzymes in eukaryotes Adds more nucleotides in the leading strand of prokaryotes Adds more nucleotides in the leading strand of eukaryotes Adds more nucleotides in the lagging strand of eukaryotes Start of replication in eukaryotes Removal of eukaryotic primer Breaks the H-bonds of bases Proof reading in prokaryotic…Choices: Origin of replication Bubble SSBP RPA Sliding Clamp PCNA DNA Pol III Pol ε Pol δ DNA Pol I RNAse H Flap 1 DNA gyrase Pol α DNA helicase Primase Single chromosome Multiple points in chromosomes DNA ligase Not applicable 12.Proof reading in eukaryotic DNA material 13.Removal of eukaryotic primer 14.Adds more nucleotides in the lagging strand of eukaryotes 15.Holds the processive enzyme in eukaryotes 16.Holds the processive enzyme in prokaryotes
- Choices: Origin of replication Bubble SSBP RPA Sliding Clamp PCNA DNA Pol III Pol ε Pol δ DNA Pol I RNAse H Flap 1 DNA gyrase Pol α DNA helicase Primase Single chromosome Multiple points in chromosomes DNA ligase Not applicable 15.Holds the processive enzyme in eukaryotes 16.Holds the processive enzyme in prokaryotesChoices: Origin of replication Bubble SSBP RPA Sliding Clamp PCNA DNA Pol III Pol ε Pol δ DNA Pol I RNAse H Flap 1 DNA gyrase Pol α DNA helicase Primase Single chromosome Multiple points in chromosomes DNA ligase Not applicable 7. Adds more nucleotides in the leading strand of prokaryotes 8. Make primers for the replicative enzymes in eukaryotes 9. Primosome in prokaryotes 10. Removal of prokaryotic primer 11. Adds more nucleotides in the leading strand of eukaryotesIdentify the enzyme/protein involved in replication: multiple choice Addition of short RNA primers at the leading and lagging stranda) dna ligaseb. dna proteinsc. dna gyrased. single-strand binding proteinse. primasef. helicaseg. 5' - 3' polymeraseh. RNA polymerasei. other:_
- You have two sewage samples that you want to check for the SARS Cov 2 viral load. Whichvariable would give you a good estimate?a. High Ct valueb. Low Ct valuec. High melting temperatured. Low melting temperaturee. None of the above The DNA fragments generated during a cycle sequencing reaction are separated by sizes usingcapillary gel electrophoresis. The determination of the actual sequence is based on detection ofa. fluorescently labelled ddNTPsb. the lengths of the fragmentsc. fluorescently labelled probesd. fluorescently labelled primers.5’ ATGCTAGACGTGTTCTAG 3’3’ TACGATCTGCACAAGATC 5’Moving from left to right, the bottom DNA strand will be read continuously.a. Tb. FA. Create the complimentary strand for the DNA strand below. Make sure to label the parts and direction of the strand. 5’-AACGGTCCAGTCCAAGTTACG-3’ 2. Below is a segment of DNA that is ready to be replicated. Outline the processes that the segment will go through during replication. Make sure to include the names of the enzymes that are involved. AATTGCCTGCTAGTCTCAG TTAACGGACGATCAGAGTC B. DNA: G T A C G C G T A T A C C G A C A T T C RNA: C A U G C G CAU A U G G C U G U A G Codons: AUG - CGC - AUA -UGG - CUG - UAA Anti-codons: UAC - GCG -UAU - ACC - GAC - AUU Amino acids: Met- Arg - Ile - Try - Leu Using the example above transcribe the following DNA strands into m-RNA and translate that strand into a polypeptide chain identifying the codons, anti-codons and amino acid sequence. 3. DNA: A T A C G A A A T C G C G A T C G C G G C G A T T C G G 4. DNA: T T T A C G G C C A T C A G G C A A T…
- Please help me to answer the following: 1. Explain how the synthesis of a DNA daughter strand growing toward a replication fork differs from the synthesis of a daughter strand growing away from a replication fork. 2. The base sequence ACGTCT represents a portion of a single strand of DNA. Please draw the complete double stranded molecule for this portion of the strand and use the representation to illustrate the replication of the DNA strand.Please clearly identify the nucleotide bases involved, the new strands formed, and the daughter DNA molecules. 3. Please explain the origin of Okazaki fragments. Thank you very muxh for your help.Please put these steps in the correct order.Is this for leading or lagging strand dsDNA pried apart. Nucleotides added on to the 3’ end of DNA. Single stranded DNA binding proteins bind. Initiator proteins bind dsDNA. RNA nucleotides are replaced with DNA nucleotides (proofreading while they go) RNA primer degraded. Primase begins replication.Match the statement to the corresponding agent/key player in DNA replication. Some items require more than one answer. choices: Origin of replication Bubble SSBP RPA Sliding Clamp PCNA DNA Pol III Pol ε Pol δ DNA Pol I RNAse H Flap 1 DNA gyrase Pol α DNA helicase Primase Single chromosome Multiple points in chromosomes DNA ligase Not applicable Proof reading in prokaryotic DNA material Joins the Okazaki fragments in leading strand Dissociates after adding the few initial nucleotides in eukaryotes Add nuclecleotides to the site of the removed prokaryotic prime Make primers for the replicative enzymes in eukaryotes Primosome in prokaryotes Proof reading in eukaryotic DNA material Holds the processive enzyme in prokaryotes Removal of prokaryotic primer