Select ALL all of the following that are true for in vivo DNA replication but NOT true for PCR: V a. The primer is made out of DNA. V b. The entire chromosome is copied. O c. Primase synthesizes the primer. V d. The new strand is built in the 5' to 3' direction. O e. Temperature is used to denature the DNA strands.

Q: Evaluation of PCR product electrophoresed on 0.8% agarose gel shows non-specific bands. The…

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Q: Polymerase chain reaction (PCR) carries out DNA replication in a test tube. Which of the following…

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Q: Which temperature is Taq polymerase's optimal temperature O 20 degrees C 72 degrees C 55 degrees C O…

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Q: Choose the one answer that fits best. Which statement regarding PCR is NOT correct (videos)?   a.…

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Q: Which of the following is an example of a recombinant DNA molecule? O a. a PCR-amplified…

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Q: Advantages of using the PCR include the following EXCEPT A it will always give the desired product…

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Q: PCR is a technique used to synthesize DNA fragments. Select all the reagents needed for PCR to…

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Q: The segment of DNA shown in the figure has restriction sites I and II, which create DNA restriction…

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Q: The purpose of using a washing soap in DNA extraction experiment is to O a. Lyse the cells to…

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Q: write two  differences between DNA replication in vivo and PCR. Draw two pictures which clearly…

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Q: Assume the samples in this diagram of gel electrophoresis are DNA molecules. Why do the samples…

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Q: You have two PCR primers: Forward- 5' TGAGCTAGGC 3' and Reverse- 5' GGTTCAGTCAG 3'. Show the binding…

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Q: The white stringy filament that appears at the end of DNA extraction experiment is a.? O a. Nucleic…

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Q: What purpose do short DNA primers serve in PCR? O They determine which sequence of DNA is copied O…

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Q: Why is DNA polymerase a required part of PCR? O It synthesizes new DNA O It synthesizes proteins O…

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Q: If your template DNA contains one copy of chromosome 16 with the Alu insertion at PV92, and one copy…

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Q: Why there is no detectable fluorescence in the samples after the first few PCR cycles? O A. Because…

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Q: What is the reason that the Taq polymerase is currently used? A the Taq polymerase is one of the…

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Q: What happens during the annealing phase of PCR? O The template DNA begins to melt. O Nucleotides are…

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Q: Why is the temperature inside the thermal cycler lowered to 55°C during the PCR process? Select one:…

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Q: Why is Taq polymerase used in a polymerase chain reaction (PCR)? O Unlike other DNA polymerases, Taq…

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Q: During the second step of PCR, the temperature is lowered in order to: denature the taq polymerase…

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Q: Suppose the DNA synthesis that occurs in PCR is less accurate than cellular DNA synthesis (as in,…

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