Choose the FALSE statement An indicator can be used to monitor a buffer range When pH equals PKa of weak acid, the buffer capacity is the greatest Polyprotic acids have multiple stoichiometric points in their titration curve At the mid-point of titration of weak acid by strong base, the pH of the solution -does not equal the pKa of the weak acid The moles of titrant equals moles of titrate

Choose the FALSE statement An indicator can be used to monitor a buffer range When pH equals PKa of weak acid, the buffer capacity is the greatest Polyprotic acids have multiple stoichiometric points in their titration curve At the mid-point of titration of weak acid by strong base, the pH of the solution -does not equal the pKa of the weak acid The moles of titrant equals moles of titrate

Biomedical Instrumentation Systems

1st Edition

ISBN:9781133478294

Author:Chatterjee

Publisher:Chatterjee

Chapter19: Biomedical Laboratory Instrumentation

Section: Chapter Questions

Problem 7P

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When dissolved in water, a(n) _____ donates H+ and a(n) ______ accepts H+. a. acid; base b. base; acid c. buffer; solute d. base; buffer
The glass electrode used in commercial pH meters gives an electrical response proportional to the concentration of hydrogen ion. To convert these responses to a pH reading, the electrode must be calibrated against standard solutions of known H+ concentration. Determine the weight in grams of sodium dihydrogen phosphate (NaH2PO4 · H2O; FW 138) and disodium hydrogen phosphate (Na2HPO4 ; FW 142) needed to prepare 1 L of a standard buffer at pH 7.00 with a total phosphate concentration of 0.100 M . See Problem 24 for the pKa values of phosphoric acid.
Calculate the pH of a buffer that contains 0.15 M MOPS anionic form and 0.25M MOPS zwitterion. The pKa is 7.2
Calculate the volume of TAE buffer that you will need to prepare a 100ml solution of 1X strength if you only have a 50X concentrated solution available.
Match the common laboratory scenarios to the correct buffer required using the guide below. Molecule pKa Value(s) Acetic acid 4.8 Carbonic acid 6.1, 10.2 Citric acid 3.1, 4.8, 5.4 HEPES 7.5 Phosphoric acid 2.1, 7.1, 12.3 TRIS 8.3 Mammalian cells require a pH of about 7.4 for cell and tissue culture experiments. Elution of remaining molecules from a cation exchange column requires washing with pH 10 buffer. Dissolution of lysozyme requires pH 3 buffer to maintain activity. Elution of remaining molecules from an anion exchange column requires washing with pH 2 buffer.…
Calculate the mole ratio of sodium acetate and acetic acid needed to make a buffer of p H 4.68 ( see Table 15.1 for Ka values or Appendices G and H for Ka and Ksp values respectively.
A small amount of NaOH is added to a weak acid buffer system. Describe what happens in terms of possible reactions and changes in pH.
The pHpH scale for acidity is defined by pH=−log10[H+] where [H+]is the concentration of hydrogen ions measured in moles per liter (M). A solution has a pH of 10.2. Calculate the concentration of hydrogen ions in moles per liter (M).
The normal range of the sodium electrolyte in the body is 134 to 145 mEq/L. The term mEq is dependent on the charge of the ion. Since sodium is a +1 ion, 134 mEq/L is the same as 134 mmol/L. An ion that has a greater positive or negative charge; however, will have 1 mEq for each positive or negative charge of the ion for every 1 mmol. For example, for Ca+2, 2mEq/1mmol. If the standard range of Magnesium in the body is 0.70 to 0.95 mmol/L, convert this value into mEq/L for the Mg2+ ion. (Use dimensional analysis to figure this out)
How much of the enzyme proteinase k (solute) is required to make 250ml of a solution with a concentration of .01mg/ml? (Weight/volume ratio)
Calculate the pH of a dilute solution that contains a molar ratio of potassium acetate to acetic acid (pKa 5 4.76) of (a) 2:1; (b) 1:3.
In your own words, explain the Mechanism of Buffer Action.