Concentration Buffer solution pka Desired ph 0.50 M Acetate 4.70 5.0 Using assigned buffer determine the buffer capacity by adding 0.1 M HCI in 1 ml portions from a burette to a 20 ml of the buffer until its pH changes as monitored with a pH meter. Record the columen added. Repeat this step using 0.1 M NaOH instead.
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- 50 mL of 5mM NaCl solution from 1 M NaCl solutionWhat is the molarity of 25.79 g of Cl2 dissolved in 500 mL of solution. (Hint: convert from grams to moles)Is there a difference between pH measurements using the pH meter and the pH paper? Compare the initial pH and the final pH of each sample. What was the most effective buffer? What was the least effective? Solución pH inicial pH final Diferencia water 7.45 2.48 0.1 M NaCl 7.28 2.03 milk 6.78 6.45 buffer de fosfato 8.76 7.78 **** Calculate the difference (subtract) the initial pH - final pH values to identify the effectiveness of each solution as a buffer. Values with little difference mean that a less drastic change occurred and are therefore more effective. On the contrary, values by much difference reflect drastic changes in pH and are less effective. What do buffers do and why are they important in biological systems?
- You have a 100X stock of a buffer. You need 100 ml of 1X buffer. How much buffer solution do you use? How much water?If 4 mL of 1 M NaOH is added to 100 mL buffer, would it still be a usable buffer according to the conventions? Explain why or why not.An aqueous solution is 3.50 % by mass hydrochloric acid, HCl, and has a density of 1.02 g/mL. The mole fraction of hydrochloric acid in the solution is ____.
- Table 2. Volume of BSA, protein content, and absorbance readings of reference solutions Solution Volume of BSA standard solution (μL) Protein content(μg/mL) Absorbance value At 595 nm 1 0 0 0 2 10 1 0.022 3 30 3 0.065 4 50 5 0.106 5 70 7 0.178 6 100 10 0.299 7 120 12 0.380 Make a graph by plotting the absorbance values versus the BSA protein content (in μg) for theseven reference solutions. When constructing the graph, be…10 mL of 0.5 M CuSO₄ * 5H₂O solution from 10 M CuSO₄ * 5H₂O solution50 mL of 15 mg/mL NaOH solution from 100 mg/mL NaOH