d) Cleavage by trypsin followed by gel-filtration chromatography gives the expected 6 products, which you sequence (shown in order of emergence from column): T-1 ETMESSAGEFGR T-2 SQTWALDHSECR T-4 TCR S-5 TME Think About It: T-5 NP e) Cleavage by Staph. aureus V8 protease followed by gel-filtration chromatography gives the expected 7 products, which you sequence (shown in order of emergence from column): S-1 RSQTWALD S-2 FGRGPQD S-3 NKTCRNP S-6 CRE T-3 GPQDNK S-7 HSE T-6 R S-4 SSAGE 1. Deduce the primary structure of this polypeptide. 2. Why would cyanogen bromide not be a good choice as a cleavage reagent? 3. Can you account for the order of elution of trypsin digest peptides from gel-filtration chromatography?
d) Cleavage by trypsin followed by gel-filtration chromatography gives the expected 6 products, which you sequence (shown in order of emergence from column): T-1 ETMESSAGEFGR T-2 SQTWALDHSECR T-4 TCR S-5 TME Think About It: T-5 NP e) Cleavage by Staph. aureus V8 protease followed by gel-filtration chromatography gives the expected 7 products, which you sequence (shown in order of emergence from column): S-1 RSQTWALD S-2 FGRGPQD S-3 NKTCRNP S-6 CRE T-3 GPQDNK S-7 HSE T-6 R S-4 SSAGE 1. Deduce the primary structure of this polypeptide. 2. Why would cyanogen bromide not be a good choice as a cleavage reagent? 3. Can you account for the order of elution of trypsin digest peptides from gel-filtration chromatography?
Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN:9781305389892
Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Chapter15: From Dna To Protein
Section: Chapter Questions
Problem 4TYK
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