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- Consider the following types of cells: F+, F-, Hfr, and F’ cells. Which of these four types of cells are capable of acting as a donor during conjugation? What genes does each cell that is capable of acting as a donor donate to the recipient cell?In a conjugation experiment, How is the time of entry determined experimentally?Compare and contrast F+ x F- and F' x F- conjugation.
- In conjugation of a Hfr cell with an F- cell: a) the container cell becomes F + b) The container cell remains F- c) The donor cell becomes F- d) The recipient cell becomes HfrIn a conjugation experiment, what is meant by the time of entry?In a conjugation experiment, what is meant by the time of entry? How is the time of entry determined experimentally?
- The sequences of the recombination sites recognized by site-specific recombinases area) Partially asymmetricb) Partially symmetricc) Symmetricd) PalindromicWhat is the DNA template of the following DNA coding: ATGGCTAACCTTGTACompare and contrast conjugation involving an F+ donor, an Hfr strain, and an F' donor.
- In Conjugation,Why a Donor TransfersDNA Directly to a Recipient?Transcribe and translate the following DNA sequence (nontemplate strand): 5’-ATGGCCGGTTATTAAGCA-3’A F- recombinant is only made after conjugation of a ________ strain with a _______, while an ______ may become a ______ if the F DNA is tranferred in its entirety. Check all that apply-- a.HFR Strain / F- / F-/ F- Recombinant b.HFR Strain / F- / F-/F+ c.HFR/ F- / F- / HFR d.F+ / F- / F-/F+ e.F+ / F- / F-/ HFR
