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Match the following Conjugation terms with the most appropriate description in the image:
F- recombinant
F- strain
F+ strain
HFR strain
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- One feature that virtually all plasmid vectors have incommon is the polylinker (also called a multiple cloningsite). Explain what a polylinker is and why it is such animportant featureWhat is meant with some plasmids carry an ampR geneBacterial plasmids often serve as cloning vectors. Describe the essential features of a plasmid vector.
- One would be correct in assuming that the restriction sites present in the multiplecloning site of the pUC18 plasmid willThe ability of F+ cells, or Hfr cells, to transfer plasmid DNA to an F- cell is properly called: transversion transformation conjugation transduction transitionTo identify mutant genes, a wild-type bacterial genomiclibrary in plasmids is used to transform the mutant bacterialstrain. A transformed ________ in which the mutantphenotype is rescued to wild type likely harbors a plasmidcontaining a wild-type copy of the corresponding gene
- A researcher has a Trp− auxotrophic strain of E. coliwith a mutation in a single gene. To identify thatmutant gene, she uses a genomic library made from a wild-type version of that same strain to find plasmids that rescue the mutant phenotype. The result issurprising. She recovers 10 plasmids that provide aTrp+ phenotype, but six of the plasmids contain geneX, while the other four contain gene Y. Our scientisthas encountered a phenomenon called multicopysuppression, related to the fact that plasmids are usually present in several copies per bacterium. Becausethe genes in the plasmids are present in more thantheir usual single copy in the bacterial chromosome,more than the usual amount of Protein X or Protein Yis being produced from the plasmids. Sometimes,overexpression of one protein can rescue the mutantphenotype caused by loss of a different protein.Suggest at least two ways that our scientist could determine which of the two genes, gene X or gene Y, actually corresponds to the…true or false if a restriction enzyme recognizes the restriction site, 5' AACGTT3', and the enzyme cuts between the second A and the C, this will produce a "sticky end," which is useful for cloning into a plasmid vector.The F plasmid encodes which two functions essential for conjugation?
- During the isolation of TOL plasmid from Pseudomonas putida, it was observed that the concentration of the plasmid DNA per 50 mL of sample was extremely low. Describe a technique you could employ to increase the concentration of the TOL plasmid.When cloning a foreign DNA fragment into a plasmid, it is often useful to insert the fragment at a site that interrupts a selectable marker(such as the tetracycline-resistance gene of pBR322). The loss of function of the interrupted gene can be used to identify clones containing recombinant plasmids with foreign DNA. With a yeast artificial chromosome (YAC) vector, it is not necessary to do this; the researcher can still distinguish vectors that incorporate large foreign DNA fragments from those that do not. How are these recombinant vectors identified?Which of the delivery techniques mentioned in Table 2 can be used to best transfer recombinant plasmids into E. coli cells