A thermophillic bacterium was isolated from a hot spring at Perak. Bernager Lee is aresearcher interested to study its amylase gene. Before the gene could be cloned for furtherwork, the genome of this bacterium should first be extracted. He plans to use the conventionalmethod utilizing Phenol, Chloroform and Isoamyl alcohol. Discuss the functions of thesethree chemicals in this procedure.

A mixture of phenol:chloroform:isoamyl alcohol (25:24:1) is added to promote the partitioning of…

Answered: Ehermophillic bacterium was isolated…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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The results of Figure 2 highlights the antibacterial activity of cacay oil. Since the method involves adding cacay oil to a "broth" medium, it can be inferred that cacay oil is hydrophilic. Agree or Not?
You discovered that a species of bacteria can break down StyrofoamTM (polystyrene) products due to anenzyme it produces, polystyrenase. You wish to study the gene that codes for this enzyme.Task 1: DNA ExtractionTo begin work on the bacterium, you begin by extracting its genomic DNA (gDNA). What is the purpose ofthe following procedures? Answer briefly but completely.a. Using sodium dodecyl sulfate, a detergentAnswer: __________________________________ b. Adding RNase A and Proteinase K during extractionAnswer: __________________________________ c. Adding ethanol before recovering the DNA extractAnswer: __________________________________ Task 2: Polymerase Chain ReactionAfter purifying the gDNA extract, you want to isolate and amplify the polystyrenase gene. You perform PCRusing the appropriate gene-targeted primers. What is the purpose of the following PCR components? Answerbriefly but completely.a. DNA polymerase isolated from Thermus aquaticusAnswer:…
A bacterium was isolated from the Pacific Ocean. Jenna is a rescarcher interested to study its amylase gene. Before the gene could be further investigated, the genome of this bacterium should first be extracted. (i) Jenna plans to use enzymes, sonication, manual grinding and liquid homogenization to achieve this objective. Elaborate in detailt these lysis methods. (ii) After the genome is obtained, explain how the anylase gene could be cloned into Escherichia coli competent cells to be amplified.
in isolating ribosomes from a yield sample, describe the ideal type of centrifugation for this separation technique based on the following:*Main type of centrifuge *Subtype centrifuge * Speed range *Operational temperature range*Type of Centrifugation
TRY TO KEEP IN SHORT AND USE OWN WORD FOR THIS QUESTION You are studying a type of bacteria isolated from the acidic water runoff of a mining operation. You subject two batches of the same bacteria type to different environmental growth conditions. One batch is grown at pH 2, while the other is grown at pH 7. All other environmental parameters are kept identical between the two batches. You then collect their proteins and run a Western blot using an antibody that binds to a proton efflux pump protein (which actively expends energy to pump protons out of a cell). How would you characterize the information obtained in this experiment? What does it tell you, and why is that potentially valuable information?
Your colleague handed you a novel strain of coli that is purifying a protein with a 6xHisTag; they claim it is superior to the TOP10 cells you have been using. But even with a larger culture size, you discover that your protein yield—using the same Ni-NTA column—is quite low. You discover that the novel strain of Escherichia coli generates an unusually high quantity of dicarboxylic acids, a byproduct of the citric acid cycle that is recognized for its ability to function as an all-purpose metal chelator. What do you think the issue is with purifying IMAC protein with this new strain of E. coli?
You have several different media onto which you inoculated eight strains of yeast (A-H). The media include a rich medium, an unsupplemented minimal medium, and minimal media each supplemented with one vitamin. Of the yeast strains, one is a prototroph and seven are auxotrophs for a vitamin. After overnight incubation, the following results were observed (tan patches represent growth): D plate 1 (A) B DE F GH plate 5 plate 4 plate 6 Which plate contains an unsupplemented minimal medium? [Select] Which plate contains a rich medium? [Select] plate 2 Which strain is a prototroph? [Select] Strain E is an auxotroph for niacin. Which plate reveals this specific auxotrophy? [ Select] plate 3 plate 7 One strain is an auxotroph for both choline and pantothenic acid. Which one is this most likely to be? [Select]
A biotechnology company discovers a bacterium isolated from waste industry that produces protease that can operate at high pH. Such protease is useful in making heavy duty detergent that has high pH catalytic condition. Thus, the company decided to produce the protease at large scale using bioreactors. a) Discuss how this is possible when the bacterium is difficult to grow and produce the protease at low level. Suggest any means to overcome these difficulties. b) Suggest what type of bioreactor and mode of culture that is suitable for the production of protease at large scale. Justify your answer.
Mannitol salt agar is often used to distinguish between different species of Staphylococcus, a gram positive bacterium that is well adapted to living on dry, salty skin. Disease-causing strains of Staphylococcus ferment mannitol; non-pathogenic strains cannot use mannitol. Is the medium Defined or Complex?
Sydney Brenner isolated Salmonella typhimurium mutants that were implicated in the biosynthesis of tryptophan and would not grow on minimal medium. When these bacterial mutants were tested on minimal medium to which one of four compounds (indole glycerol phosphate, indole, anthranilic acid, and tryptophan) had been added, the growth responses shown in the following table were obtained. Mutant Minimal medium Anthranilic acid Indole glycerol phosphate Indole Tryptophan trp-1 − − − − + trp-2 − − + + + trp-3 − − − + + trp-4 − − + + + trp-6 − − − − + trp-7 − − − − + trp-8 − + − − + trp-9 − − − − + trp-10 − − − − + trp-11 − − − − + Give the order of indole glycerol phosphate, indole, anthranilic acid, and tryptophan in a biochemical pathway leading to the synthesis of tryptophan. Indicate which step in the pathway is affected by each of the mutations.
Sydney Brenner isolated Salmonella typhimurium mutants that were implicated in the biosynthesis of tryptophan and would not grow on minimal medium. When these bacterial mutants were tested on minimal medium to which one of four compounds (indole glycerol phosphate, indole, anthranilic acid, and tryptophan) had been added, the growth responses shown in the following table were obtained. Mutant Minimal medium Anthranilic Indole glycerol acid Indole Tryptoph phosphate trp-1 trp-2 trp-3 trp-4 trp-6 trp-7 trp-8 trp-9 trp-10 trp-11 - Give the order of indole glycerol phosphate, indole, anthranilic acid, and tryptophan in a biochemical pathway leading to the synthesis of tryptophan. Indicate which step in the pathway is affected by each of the mutations. + 1 + 1 II
When present on the leaves of plants, the bacterium Pseudomonas syringae can promote frost damage to plants. Mutant strains, lacking the “ice” gene, have been applied to plants to try and protect the plants from wild-type P. syringae-induced frost. Assume that I am telling the truth that wild-type P. syringae nucleates ice formation at -2 °C, and provide an explanation as to why application of this altered (mutant) bacterium to plants might be a beneficial agricultural strategy in areas where morning lows occasionally dip down to 28-30 °F.

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