4. Two chemical reactions also contribute to the generation of theelectrochemical gradient. What are the reactions, where do theyoccur, and why do these factors affect the gradient?5. The electrochemical gradient can be utilised by ATP synthaseto generate ATP - explain how the location and structure of ATPsynthase enable it to perform this function.e) The light reactions of photosynthesis generate ATP andNADPH for use in the dark reactions (i.e., the Calvin cycle).Why are they needed?

Electrochemical Radiant always consists of two gradients. First one is electrical gradient and the…

Answered: electrochemical gradient. What are the…

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Biology

electrochemical gradient. What are the reactions, where do they occur, and why do these factors affect the gradient?

Biology (MindTap Course List)

11th Edition

ISBN:9781337392938

Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Publisher:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Chapter9: Photosynthesis: Capturing Light Energy

Section9.2: Chloroplasts

Problem 2C: What is the significance that the combined absorption spectra of chlorophylls a and b roughly match...

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Concept explainers

Osmosis

In simple words, osmosis is the slow, gradual movement of a liquid through a membrane. But there are nuances to this process. For instance, there has to be an imbalance in the distribution of solute in the liquid on either side of the membrane, which has to be semipermeable. The liquid in the region with a lower solute concentration moves to the region with a higher concentration, thereby displacing the solution in the process. The objective is to equalize the concentration of molecules on both sides of the membrane; the displacement of liquid can be viewed as a byproduct of this equalization. Since there is no energy required to carry osmosis, it is considered a passive process.

Question

4. Two chemical reactions also contribute to the generation of the
electrochemical gradient. What are the reactions, where do they
occur, and why do these factors affect the gradient?
5. The electrochemical gradient can be utilised by ATP synthase
to generate ATP - explain how the location and structure of ATP
synthase enable it to perform this function.
e) The light reactions of photosynthesis generate ATP and
NADPH for use in the dark reactions (i.e., the Calvin cycle).
Why are they needed?

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2.) A.) Explain how electron transfer leads to ATP biosynthesis in the light reactions of photosynthesis. You must give details about how electron flow builds a pH gradient and a description of the compartments in the chloroplast in relation to pH. Also, compare the ETC of the mitochondrion with the ETC of the light reactions for both the source and destination for electrons. B.) Would ATP biosynthesis happen if ionophores, which would create holes in the thylakoid membrane and allow uncontrolled passage of charged molecules such as protons between compartments, are provided to the chloroplast? Why or why not? C.) How would you modify Complex I of the electron transport chain to decrease ATP yield from Step 3 of β-oxidation? As part of this, you must explain why there is a difference in ATP yield between NADH and FADH 2 AND give a specific change to the ETC that would decrease ATP yield from Step 3
5. a) The cell creates molecules of NADH and FADH2to use in the electron transport chain as they are electron carrier molecules. The electron transfers from these moleculesdrives the movement of what molecule across the mitochondrial membrane?b) What is chemiosmosis?c) How does the cell use chemiosmosis to drive overcome the thermodynamic barrier of bringing phosphates close togehter to create ATP?d) What steps of oxidative phosphorylation are in the cytosol and which are in the mitochondria?e) Cellular respiration can be controlled at the post-translational level. What does this mean, using GLUT as an example? Why is post-translational control of GLUT evolutionarilyadaptive over transcriptional control?f) How does establishment of equillibria of various molecules control metabolic reactions? What are three ways high ATP in the cell control cellular respiration?g) Why are the enzymes controlling the initial steps of various biochemical pathways are targeted by allosteric control?
1. A) For the schematic of a chloroplast shown in the image attached, match the major input and output molecules of photosynthesis to the letters shown. Molecules: CO2, H2O, O2, sugar A: B: C: D: B) In the image attached, the light reactions are shown to take place in the thylakoid. Where specifically do these reactions take place? C) Where does the Calvin cycle take place?
Protons move across the thylakoid membrane in both directions during the light reactions of photosynthesis...... 1. When protons move in to the thylakoid, are they moving up or down their concentration gradient? (From low to high concentration or from high to low concentration?) 2. Since protons are charged, they cannot freely diffuse across a plasma membrane. What protein(s) facilitate the transport of protons in to the thylakoid?
7. Why are electron carriers (NAD+/NADH and FAD/FADH2) so important in the process of cellular respiration? a) They deliver electrons to the ETC, which in turn sets up chemiosmosis, where most of the ATP is generated. b) They separate the electrons from the protons so that the protons can be moved out of the mitochondrion. c) NADH and FADH2 are major components of the ETC, so without them, there would be no ETC in the cell. d) The electrons that they carry are able to directly phosphorylate ADP in order to generate the bulk of ATP in the cell. e) They transport protons across the mitochondrial membrane.
1. Why is water split by PSII in the light reactions? 2. How is ATP synthesis powered in the light reactions? How does this compare to the oxidative phosphorylation of Cellular Respiration?
Thylakoids were isolated from chloroplasts and incubated in the dark in an acidic solution (pH 4) to equilibrate the pH. After 30 minutes, the thylakoids were transferred to a basic solution (pH 8) and kept in the dark. Will this system produce ATP? Explain. Will this system produce G3P? Explain.
A suspension of inside-out submitochondrial particles (i.e., the F1 unit is on the outside of the particle) is placed in a solution that contains ADP, Pi, and NADH. Will increasing the proton concentration of the solution result in ATP synthesis? Explain.
1. a) Describe what is happening in ATP Synthase in plain English! (how would you describe this step for someone who hasn’t taken Biol) b) If someone wanted to perform an experiment to evaluate the output of a particular step, which variable would be the best option to measure? (Think about which variable is UNIQUE to each step!)
The Relative Efficiency of ATP Synthesis in Noncyclic versus Cyclic Photophosphorylation If noncyclic photosynthetic electron transport leads to the translocation of 7 H+/2e- and cyclic photosynthetic electron transport leads to the translocation of 2 H+/e-, what is the relative photosynthetic efficiency of ATP synthesis (expressed as the number of photons absorbed per ATP synthesized) for noncyclic versus cyclic photophosphorylation? (Assume that the CF1CF0-ATP synthase yields 3 ATP/14 H+.)
Describe the flow of electrons through photosystems I and II in the noncyclic electron transport pathway and the products produced. Contrast this flow with cyclic electron transport.
Figure 27.3 illustrates the response of R (ATP-regenerating) and U (ATP-utilizing) enzymes to energy charge. a. Would hexokinase be an R enzyme or a U enzyme? Would glutamine: PRPP amidotransferase, the second enzyme in purine biosynthesis, be an R enzyme or a U enzyme? b. If energy charge = 0.5: Is the activity of hexokinase high or low? Is ribose-5-P pyrophosphokinase activity high or low? c. If energy charge = 0.95: Is the activity of hexokinase high or low? Is ribose-5-P pyrophosphokinase activity high or low?