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EXPERIMENT
Determination of Temporary and Permanent Hardness of Water.
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- Internal standard (As , Cs ), analyte with known concentration (Ax , Cx ) In a chromatographic equipment, a solution containing 0.006727 X and 0.008331 M S give peak area of Ax= 2316 and As= 207. To analyze an unknown sample, 5.0mL of 0.008331M S was added to 5.0mL of X, and the mixture was diluted to 50.0mL. This mixture gave a chromatography spectrum with area Ax= 3206 and As= 200. Find Cx.Two components in an HPLC separation have retention times thatdiffer by 22 s. The first peak elutes in 10.5 min and the peakwidths are approximately equal. Use a spreadsheet to find theminimum number of theoretical plates needed to achieve thefollowing resolution, Rs, values: 0.50, 0.75, 0.90, 1.0, 1.10, 1.25,1.50, 1.75, 2.0, and 2.5. How would the results change if peak 2were twice as broad as peak 1?The detecton limits reported for Al with ICP-AES and GF-AAS are 2 ppb and 5 picograms, respectivly. If we assume that a 20 microliter sample volume is used for the GF-AAS determination, which method has the lower detection limit? (1 picogram = 10-12 gram.)
- Preparing a standard curve. 1. determine the absorbance max of [FeSCN]2+ it is 448.1 2. determine the concentration of FeSCN2+ in the stock solution. M1V1=M2V2 stock solution: ~0.200 M iron (iii) nitrate in 1M nitric acid intial volume: 0.3mL final volume: 10.3mL volume: 10mL ~0.002M potassium thiocyanate initial volume: 0.3mL final volume: 10.3mL volume: 10mL not sure if this is needed stock solutions: ~0.200M iron (iii) nitrate in 1M nitric acid: 0.207M ~0.002M iron (iii) nitrate in 1M nitric acid: 0.00209M ~0.002M potassium thiocyanate: 0.00193MA graphite furnace atomic absorption spectrophotometer (AAS) was used to determine the arsenic (As) concentrations in drinking water. A large set of blank samples yielded an average blank signal of 0.013 and a blank standard deviation of 0.0030. The following data were obtained for a calibration curve. [As] (ppm) 2.0 4.0 6.0 8.0 10.0 Absorbance 0.17 0.28 0.48 0.53 0.72 Using a spreadsheet on an Excell, create a graph of the data using symbols only for the data points. Plot the line on the same graph and include the graph to this question. (a). What is the sensitivity of the method? (b). Calculate the LOD and LOQ for As. (c). A sample of water yielded an absorbance of 0.43. What is the concentration of As in the water?Retention time is the time taken for the mobile phase to reach the detector. True False
- A spectrophotometric method for the quantitative determination of the concentration ofPb2+ in blood yields an Ssamp of 0.133 for a 1 mL sample of blood that has been diluted to 6 mL. A second sample is spiked with 1 µL of a 1467 ppb Pb2+ standard and diluted to 6 mL, yielding an Sspike of 0.491. Determine the concentration of Pb2+ in the original sample of blood.1 mL was taken from the sample filtrate and mixed with 13 mL pure water and 4 mL sulfomolybdic acid and 2 mL dilute SnCI2 solution by adding it, and after waiting for 15 minutes, the absorbance of the resulting solutions against pure water was read at 520 nm. if the function of the calibration graph obtained with standard phosphorus solutions of 0.5-2.5 mg/mL is y= 0.245x + 0.107 and the absorbance of the serum sample is 0.342, how many grams of phosphorus is the amount in the sample?Concentration=2.5 X 10^3 μM OD400nm=0.859 1cm cuvette is used what is the extinction coefficient (μM^-1 cm^-1)
- Column Plate Number: 16Retention Factor: 3.1Sample Peak Retention Time: 1.2minutesSample Peak Width: 1.53First Peak Retention Factor: 2.10Second Peak Retention Factor: 1.37Efficiency: 0.43Pressure: 375barrColumn: 28cm x 5mmViscosity: 2.3poiseFlow Rate: 1.2Column Permeability: 2.8 FIND: a & ba. Resolution b. Pressure if flow rate is adjusted to 2.3 c. Particle Size d. Selectivity Factor e. The retention time of the unretained peak1. Calculate: Retention factor (k), Separation factor (α), and Resolution (R) for slide 29. Comment on how these may be adjusted to give optimum values.In HPLC, fill in the blanks with the choices below: - chromatogramretention timevoid volume markernumber of theoretical platespeak widthplate height Resolutioncapacity factorreduced retention volumeselectivity - 1. This chromatographic parameter is a measure of efficiency ____.2. A high value of the _______ means high efficiency.3. ______ is both a measure of selectivity and efficiency.4. To measure the void time a _______ is use.5. A higher value of _______ means higher HETP.6. Another term for retention factor is _______7. _______ is ability of the chromatographic system to discriminate differentanalytes.8. The plot of the detector response vs. time is called a _______9. _______ distance of the peak maxima from the injection point expressed intime units.10. The difference between the retention volume and the void volume is called _____