Explain the correlation between the structure of the compounds (b-Carotene, chlorophyll ) and the polarity of the solvent (hexane,70/30 hexane/acetone) that separated the compounds on the column. (TLC plate experiment on spinach)
Q: Show calculations for making 1X TAE gel running buffer and 1.25% agarose gel for your experiment.
A: Buffer is a solution that tends to maintain pH of the solution when acid or base is added to it. Per...
Q: 12.45 What is osmosis? What is a semipermeable membrane?
A: Colligative properties: The properties which are based on number of solute particles but not on t...
Q: 2. A quart of water weighs 4.05 pounds Calculate: a. Mass in slugs b. Mass in kilograms С. Volume in...
A: Given mass in pound
Q: reaction was monitored as a function of time: AB→A+B A plot of 1/[AB] versus time yields a straigh...
A: AB-->A + B Slope = 0.052 mol.L^-1.s^-1 Initial concentration = 0.52 mol.L^-1 Final concentratio...
Q: Which of the following statements is TRUE about elimination tests for anions? Select one: O a. NO; i...
A: Anionic analysis refers to identification of anions present in the salt mixture. Various anions pres...
Q: 1. How many molecules of oxygen are in 50.0 g of oxygen?
A:
Q: Part B 8.10x10 kJ/mol Submit Request Answer • Part C 1860 kJ/mol t
A: E= hc/wavelength
Q: What is the molar solubility of NiS if the Ksp is 3.0 × 10-20? NiS(s) ⇌ Ni2+(aq) + S2−(aq)
A: Given, NiS(s) ⇌ Ni2+ (aq) + S2- (aq) The Ksp of NiS is 3.0× 10-20 The molar solubility of NiS is:...
Q: Be sure to answer all parts. Heating copper(II) oxide at 400°C does not produce any appreciable amou...
A:
Q: What is the difference of using toluene, hexane, and dichloromethane as solvents in SN2 reactions? ...
A:
Q: What is the pOH of 2.60 M solution of hydroiodic acid?
A:
Q: Ammonium perchlorate (NH,CIo,) is the solid rocket fuel used by the U.S. Space Shuttle. It reacts wi...
A: Mass of NH4ClO3 = 4.0 g MW of NH4ClO3 = 117.5 g/mol
Q: How many protons, neutrons, and electrons are there in a neutral atom of 113In (indium-113)?
A: Atomic number of indium = 49 Atomic mass of indium = 113
Q: Priestley prepared oxygen by heating mercury(II) oxide. From the data given below estimate the tempe...
A: Goven-> ∆H°=90.83 KJ =90830 J S°(Hg) = 76.02 J/mole.K S°(HgO) = 70.29J/mole .K S°(O2) = 205.0 J/...
Q: What is the minimum concentration of phosphate ions required to precipitate silver phosphate in a 0....
A: Molarity of AgNO3 = 0.010 M Ksp for silver phosphate = 2.83 × 10^-18
Q: Q3/ A/ What is the concentration and density of a solution in mol/L when 80 g of calcium carbonate, ...
A:
Q: Enter the number of electrons in each energy level (shell) for each of the elements. If the energy l...
A: Enter the number of electrons in each energy level (shell) for Hydrogen element.,
Q: Complete the following acid-base reaction and write the complete ionic equation for the reaction: (Y...
A: Acid-Base reactions are those reactions in which an acid reacts with base to form salt and water. Ac...
Q: 12.49.Explain why it is essential that fl uids used in intravenous injections have approximately the...
A: Answer of 12.49 is given below
Q: Which of the following is NOT true of the transition state? O a. It is unstable and dissociate into ...
A:
Q: 5. Consider the phase diagram of Cu-Be shown below. At 1000°C with an overall alloy composition of 1...
A: Given phase diagram:
Q: the use of an indicator to determine a concentration using titration as good as using a pH meter? W...
A: Principle of pH meter - it is scientific instruments which measure hydrogen ion activity in so...
Q: Assuming 100% dissociation, 1.0 mol of NaBr dissolved in 250 g of water [ Select] have the same coll...
A:
Q: How many subshells are in the n = 3 shell? subshells How many orbitals are in the n = 3 shell? orbit...
A: given :- n =3 (n is the principle quantum no.) To find :- number of subshells number of orbitals...
Q: If a plot of 1/[A] versus time produces a straight line with a positive slope for the reaction A B +...
A:
Q: A chemist measures the amount of chlorine gas produced during an experiment. She finds that 1.35 g o...
A: This question is related to stoichiometry which determines the quantitative relation between reactan...
Q: If you calculated the molar mass of your unknown liquid to be 70.1 g/mol, which of the following do ...
A: Given-> Calculated molar mass of unknown liquid =70.1 gm/mole.
Q: 1. Carbon tetrachloride with a mass of 450 kg is placed in a container with 0.325 m³ in volume. Calc...
A: A question based on general chemistry that is to be accomplished.
Q: A 0.027 M solution of NaY is made and the pH measured at equilibrium. If Y is a weak base and if the...
A:
Q: Consider the following reaction carried out a 1000 K: A (g) B (g) + C2 (g) Kp = 0.1000 a. At 1000 K ...
A: a.) To calculate the partial pressure , we would draw an ICE table and use given Kp value and Initia...
Q: Given the standard heats of formation for the following compounds, calculate AH 208 for the followin...
A:
Q: Why is there a need for buffers? Please explain in depth and detail
A: A buffer solution is a solution that can withstand changes in pH on addition of acidic or basic com...
Q: Which of the following is the characteristic of London dispersion forces? A. The electron cloud of ...
A: London dispersion forces : It is a kind of intermolecular forces which results form formation of dip...
Q: Decide whether each molecule or polyatomic ion is polar or nonpolar. If the molecule or polyatomic i...
A:
Q: ) Febrz + kzC0s 5) K2 C03+ Baliz CO(OH)3 + HNO3 + > #Cl and KOH to 9 HBr and Fe(OH)s ->
A: According to the Law of conservation of mass " all atoms of different elements must be equal on both...
Q: The decomposition of XY is second order in XY and has a rate constant of 7.00×10−3 L mol−1 s−1 at a ...
A:
Q: Calculation of Phosphate Buffer Solution using Henderson-Hasselbach equation
A: Henderson-Hasselbach equation is given as: pH = pKa + log [Conjug...
Q: Decide whether each molecule or polyatomic ion is polar or nonpolar. If the molecule or polyatomic i...
A: ★ F2 : F2 is homonuclear diatomic molecule which is non polar . A homonculear diatomic molecule F2 ...
Q: ACT 3 Colligative Properties of Nonelectrolyte Solutions Review Questions 12.39 What are colligative...
A: Vapour pressure - The pressure exerted by the vapour in the equilibrium with liquid is called as v...
Q: An unknown compound has the following chemical formula: NO where stands for a whole number. Measurem...
A: Moles of nitrogen = 8.4 mole Moles of oxygen = 8.43 mole Chemical formula = NxO To find :- Value of...
Q: An unknown compound has the following chemical formula: NO, where x stands for a whole number. Measu...
A: Given :- chemical formula of unknown compound = NOx no.of moles of O = 5.1 mol no.of moles of N =...
Q: A toy top has a mass of 32.0 g CU. How many atoms CU are in this spinning top, given that the avera...
A: Given :- mass of Cu at the toy top = 32.0 g Average atomic mass of Cu = 63.55 g/mol To...
Q: We have assumed [CO32-] << [HCO3] in our precedent calculation and accordingly ignored the [CO3²-] c...
A: The common pH of rain water is 5.5 to 8.5 We need to explain that for most natural water, [CO32-] &l...
Q: A solution contains 1.01x10-2 M silver acetate and 8.49x10-3 M iron(II) nitrate. Solid potassium hyd...
A:
Q: Which combination of AG° and K is possible at standard conditions? 1. AG° = -114 J, K = 0.0053 2. AG...
A:
Q: what is the number of moles of calcium carbonate reacted?
A:
Q: Question 2 Assuming the dopant atom occupies the Ga-site. The doping of GaAs with Si gives (a) N-typ...
A: Semiconductors can be defined as the materials whose conductivity lie between conductors and insulat...
Q: Which of the following processes is exothermic, given the following: N2(g) + 2 02(g) N204(1) AH°=9.6...
A: We know for exothermic reaction standard enthalpy of the reaction must be negative. So we will see f...
Q: I have to answer the following questions relating to the attached HNMR spectrum. a. Match the hydrog...
A: A question based on NMR spectroscopy that is to be accomplished.
Q: Lactic acid has a K. of 2.502 x 103. A solution contains 0.275 M lactic acid and 0.375 M sodium lact...
A: Buffer solution: The mixture of lactic acid and sodium lactate act as a buffer solution. The solutio...
Explain the correlation between the structure of the compounds (b-Carotene, chlorophyll ) and the polarity of the solvent (hexane,70/30 hexane/acetone) that separated the compounds on the column. (TLC plate experiment on spinach)
Step by step
Solved in 2 steps with 1 images
- Explain briefly the differences in Rf values of the pigments that you have separated from spinach juice. What properties must the pigment have to travel further in the chromatogram? Consider the structures of cholesterol and ?-carotene. Which do you think will have the highest Rf value if the solvent used was acetone - hexane (20:80)? Why?Draw a chromatogram showing the spots when a mixture of the following compounds is separated using water-acetone as the mobile phase in a cellulose-based stationary phase. The following compounds have Rf values of 0.8, 0.6,0.3.There have been cases where wine has been mistakenly adulterated with ethylene glycol (,2-ethanediol, bp760 97.6 ˚C). As a forensic chemist, suggest how would you use GLC to prove conclusively that ethylene glycol was actually present?
- Based on the resulting chromatograms after visualization with iodine vapor, which between the solvent systems WHITE and MIX resulted in a more effective separation of the pigments of the indigo extract? Explain your answer in terms of the solvent that did not effectively separate the components of the root extract.Consider the structures of cholesterol and ?-carotene. Which do you think will have the highest Rf value if the solvent used was acetone - hexane (20:80)? Why?Briefly outline why cinnamaldehyde, cinnamic acid and cinnamyl alcohol travelled different distances on the TLC plate. The solution used to develop the TLC plate was a mixture of toluence and methanol (8:1.5)
- Calculate the weight of nitrobenzene that codistills (bp 99°C) with each gram of water during a steam distillation. You may need the data given.Which of the following carotenoid structures below would you expect to have the lowest RF in a chromatography experiment using an ether-petroleum ether mobile phase? Why? Lycopene: β-carotene: Vitamin AYou performed an isocratic reversed-phase HPLC separation of phenol, toluene and catechol using a DAD detector set at 275nm and obtained the chromatogram shown below. What are the polarities of the mobile phase and stationary phase in this separation? What order would you expect these compounds to elute in? Why? What changes could you make to the mobile phase to decrease the elution time of the last compound and improve its peak shape? If all three species are present in the analyzed solution at the same concentration, what might account for different in peak height for the first two compounds eluting from the column? What change to the instrument set-up could you make to increase the detector response of this analyte?
- Cis-3-hexene and trans-3-hexene can be separated and analyzed using GC without injecting an equimolar standard to a high accuracy. Explain why that is the case for these two compounds while we had to inject an equimolar solution for our experimentSilica gel is very polar stationary phase. Therefore, more polar molecule sticks to silica gel on the TLC plate and has higherRfvalue. True FalseImagine that the blue component of a given mixture moved 2.5 cm from the oigin or starting line, and the solvent had traveled 10.0cm, What is the Rf for the blue dye A .4.4 B 0.25 C. 7.5 D. 25