First image contains the respective absorbance readings of the samples specified in the 96-well plate set-up in the 2nd image . The absorbance reading that needed to be write should be the average absorbance of 12 - replicate analysis (i.e. Calculate the average of the absorbance of A1 to A12 to get the final absorbance of the "blank". ThermoSCIENTIFICPlateSession3*595 nm P20.3°CMULTISKAN GO2356Z89101112A 0.044 0.045 0.045 0.048 0.050 0.043 0.047 0.046 0.048 0.048 0.052 0.0460.139 0.132 0.135B 0.135 0.141 0.138C 0.155 0.160 0.169D 0.219 0.230 0.2140.138 0.137 0.137 0.145 0.136 0.1360.162 0.164 0.152 0.157 0.156 0.1660.221 0.213 0.216 0.230 0.239 0.2140.155 0.164 0.1590.215 0.232 0.222E 0.267 0.242 0.274 0.263 0.260 0.272 0.274 0.269 0.243 0.266 0.277 0.249F 0.279 0.293 0.283 0.276 0.284 0.287 0.293 0.291 0.280 0.287 0.294 0.2790.160G 0.158 0.1670.161 0.166 0.162 0.155 0.163 0.161.H 0.143 0.1230.123 0.120 0.127 0.121 0.121 0.123 0.1350.158 0.166 0.1570.125 0.121 0.117Export2:29AMSave AsClose ABCDEFGH1BlankStandard1Standard2Standard3Standard4Standard52BlankStandard1Standard2Standard3Standard4Standard53BlankStandard1Standard2Standard3Standard4Standard5ProteinProteinProteinSample ofSample of Sample ofUnknownUnknownUnknownConcentration Concentration Concentration4Blank BlankStandard1Standard2Standard3Standard4Standard55ProteinSample ofUnknownConcentrationStandard1Standard2Standard3Standard4Standard56Blank Blank BlankStandard Standard11Standard2Standard37Standard4Standard5Standard2Standard3Standard48Standard5Standard1Standard2Standard3Standard4Standard59BlankStandard1Standard2Standard3Standard4Standard5ProteinSample ofProteinSample ofUnknownProteinSample ofUnknownProteinSample ofUnknownProteinSample ofUnknownUnknownConcentrationConcentration Concentration Concentration Concentration Concentration10BlankStandard1Standard2Standard3Standard4Standard511BlankStandard1Standard2Standard3Standard4Standard5ProteinSample ofUnknownProteinSample ofUnknownConcentration Concentration12BlankStandard1Standard2Standard3Standard4Standard5ProteinSample ofUnknownConcentration

Protein concentration of unknown sample could be calculated by plotting the graph for standard…

First image contains the respective absorbance readings of the samples specified in the 96-well plate set-up in the 2nd image . The absorbance reading that needed to be write should be the average absorbance of 12 - replicate analysis (i.e. Calculate the average of the absorbance of A1 to A12 to get the final absorbance of the "blank".

First image contains the respective absorbance readings of the samples specified in the 96-well plate set-up in the 2nd image . The absorbance reading that needed to be write should be the average absorbance of 12 - replicate analysis (i.e. Calculate the average of the absorbance of A1 to A12 to get the final absorbance of the "blank".

Human Physiology: From Cells to Systems (MindTap Course List)

9th Edition

ISBN:9781285866932

Author:Lauralee Sherwood

Publisher:Lauralee Sherwood

Chapter17: Energy Balance And Temperature Regulation

Section: Chapter Questions

Problem 3RE

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