You measure Protein A and Protein B to have a Kd of 10-8 M. At what concentration of Protein B will Y be: a) 0.1 b) 0.3 c) 0.5 d) 0.7 e) 0.9 f) You decide to measure a kinetic parameter as well, and determine the rate association constant to be 5.2 x106 M-1s-1. What is the dissociation rate constant? Be sure to include the proper units. g) From the dissociation rate constant you calculated, determine the half-life of the complex between Protein A and Protein B by using the equation: t1/2 = (In2)/kd

Biochemistry
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ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
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Chapter1: Biochemistry: An Evolving Science
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3) You measure Protein A and Protein B to have a Kd of 10-8 M. At what concentration
of Protein B will Y be:
a) 0.1
b) 0.3
c) 0.5
d) 0.7
e) 0.9
f) You decide to measure a kinetic parameter as well, and determine the rate
association constant to be 5.2 x106 M-¹s-1. What is the dissociation rate
constant? Be sure to include the proper units.
g) From the dissociation rate constant you calculated, determine the half-life of
the complex between Protein A and Protein B by using the equation:
t1/2 = (In2)/kd
Transcribed Image Text:3) You measure Protein A and Protein B to have a Kd of 10-8 M. At what concentration of Protein B will Y be: a) 0.1 b) 0.3 c) 0.5 d) 0.7 e) 0.9 f) You decide to measure a kinetic parameter as well, and determine the rate association constant to be 5.2 x106 M-¹s-1. What is the dissociation rate constant? Be sure to include the proper units. g) From the dissociation rate constant you calculated, determine the half-life of the complex between Protein A and Protein B by using the equation: t1/2 = (In2)/kd
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