Following 'salting out' experiments with crude samples, you now have 5 proteins, named after your friends, in your sample. You want to further purify your proteins in solution (pH 7.6). Their characteristics are given in the table below. Protein names Annie Molecular weight (Da) 46,000 11,500 27,500 31,000 200,000 pl 6.2 Bob 10.4 Chris Dan 6.9 4.5 Emily 8.6 If you separate this sample by SDS-PAGE (the sample was loaded at the top), which of the following proteins would you expect to find at the bottom of the gel? A. Annie B. Bob O C. Chris O D. Dan O E. Emily

Biochemistry
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ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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Following 'salting out' experiments with crude samples, you now have 5 proteins, named after your friends, in your sample. You want to further purify your
proteins in solution (pH 7.6). Their characteristics are given in the table below.
Protein names
Molecular weight (Da)
46,000
11,500
27,500
31,000
200,000
pl
6.2
Annie
Bob
10.4
Chris
6.9
Dan
4.5
Emily
8.6
gel?
If you separate this sample by SDS-PAGE (the sample was loaded at the top), which of the following proteins would you expect to find at the bottom of the
A. Annie
В. Bob
C. Chris
D. Dan
O E. Emily
Transcribed Image Text:Following 'salting out' experiments with crude samples, you now have 5 proteins, named after your friends, in your sample. You want to further purify your proteins in solution (pH 7.6). Their characteristics are given in the table below. Protein names Molecular weight (Da) 46,000 11,500 27,500 31,000 200,000 pl 6.2 Annie Bob 10.4 Chris 6.9 Dan 4.5 Emily 8.6 gel? If you separate this sample by SDS-PAGE (the sample was loaded at the top), which of the following proteins would you expect to find at the bottom of the A. Annie В. Bob C. Chris D. Dan O E. Emily
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