From an extract of human cell growing in tissue culture, A fibrous substance was obtained. How would you distinguish whether it was DNA or RNA?
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- How many kilobases of the DNA strand below will code for the protein product?If the genetic code used 4 bases at a time, how many amino acids could be encoded?DNA contains many hydrogen bonds. Are hydrogen bonds stronger or weaker than covalent bonds? What are the consequences of this difference in strength?
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- One common type of non-homologous end joining, to repair a double stranded break, occurs when enzymes cut out a few nucleotides around the break, and then fuse the ends back together true or false?You have used the technique of chromatin immunoprecipitation to isolate DNA fragments containing a DNA-binding protein of interest. Suppose that you wish to know whether a particular known DNA fragment is present in the isolated mixture. How might you detect its presence?Suppose a piece of DNA is cut into four fragments of varying sizes. Where would you expect to find the larger fragments on the gel (top, middle, or bottom)? Why? Suppose you had 1000 pieces of each of the four fragments, how many bands would appear on the gel?