Gal4–VP16 requires SAGA for activation. Strains containing the Gal4+, Gal4–VP16, or Gal4–VP16–F442A activators were tested for their ability to grow on glucose and galactose as carbon sources and for their dependence on Spt20 for activation. Strains were tested for growth on SC-Leu plates containing glucose or galactose by replica plating and are shown after 4 d of growth at 30°C. The SPT20 allele and the activator present in each strain is shown in the figure. a) Describe the strain Spt20+Gal4Δ (Fig. 5B above). Is this strain able to activate the Gal genes? Why or why not? b) What is VP16-FA?
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
Gal4–VP16 requires SAGA for activation. Strains containing the Gal4+, Gal4–VP16, or Gal4–VP16–F442A activators were tested for their ability to grow on glucose and galactose as carbon sources and for their dependence on Spt20 for activation. Strains were tested for growth on SC-Leu plates containing glucose or galactose by replica plating and are shown after 4 d of growth at 30°C. The SPT20 allele and the activator present in each strain is shown in the figure.
- a) Describe the strain Spt20+Gal4Δ (Fig. 5B above). Is this strain able to activate the Gal genes? Why or why not?
- b) What is VP16-FA?
![B
glucose
galactose
SPT20
GAL4*
SPT20* SPT20*] SPT20*
VP16+
gal4A
VP16-FA
spt204 spt20A spt20A
GAL4* |VP16+
VP16-FA
spt20A
gal4A](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F636f1fa6-82ec-49a8-aed7-ba1fe96f9a43%2F269b2f39-e8b0-4023-b7c4-e7e6eb3a4de5%2Fgqg4z4c_processed.png&w=3840&q=75)
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