Given below is the block diagram of gas chromatography. Identify the unmarked component Column Gas Supply Microprocessor Recorder Detector Select one: a. Flow regulator b. Sample injection system c. Pressure regulator
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- In order to execute paper chromatography, which of the following stages is required?I. Load the standard/s beside the sample spotsII. Allow the chromatogram to touch sides of beakerIII. Saturate the chamber with vapor of the mobile phaseIV. Place the spots at the same height as the solvent levelplease quickly ,within 1hour thanks ! If it takes longer than an hour, i don't need the answer (2) List the representative stationary phase for gas chromatography.What are the general characteristics of Gas Chromatography, Thin Layer Chromatography, Ion Exchange, HPLC, SEC and Affinity Chromatography? What elutes first? Additionally can you explain what exactly is stationary phase and mobile phase?
- separate a mixture containing acetone, acetamide and 1,2-dichloroethane using a high- performance liquid chromatography (HPLC) technique. The instrument was equipped with a 40-cm column packed with cyano propyl bonded to the siloxane backbone material. i) List THREE (3) examples of mobile phase solvent/solvent systems that can be used with the column above. ii) Determine the sequence in which the three analytes eluted from the column and explain the underlying principle applied. iii) Sketch the chromatogram that would be produced if the column was replaced with a column filled with C18 hydrocarbon bound to the siloxane backbone material and acetonitrile was employed as the mobile phase. Label the peaks observed.The following data give the recovery of bromide from spiked samples of vegetable matter, measuredusing a gas–liquid chromatographic method. The same amount of bromide was added to each specimen(Roughan, J.A., Roughan, P.A. and Wilkins, J.P.G., 1983, Analyst, 108: 742).Tomato: 777 790 759 790 770 758 764 ug/gCucumber: 782 773 778 765 789 797 782 ug/g(a) Test whether the recoveries from the two vegetables have variances which differ significantly.(b) Test whether the mean recovery rates differ significantly.Draw a schematic diagram of how a Flash Column Chromatography works? Please answer at your own words
- Brief discussion of column chromatographic separation (solvent polarity, elution ofcompounds etc.)I) Sketch the apparatus set up for the chromatographic technique used byIman. Use the correct chromatographic terminologies to label all of theapparatus, materials, and phases. II)Predict the sequence of elution when the mixture was loaded onto theseparation column. Justify your answer. III) After the first compound was eluted, Iman discovered that the secondcompound was taking too long to pass through the column. Please suggestany strategies that could be used to aid the elution process. Justify youranswer.Complex chromatographic patterns can be simplified bypassing the components emerging from the gas chromatographic column through a(n) ___________.
- As a newly appointed research officer, Marissa has been assigned a task to separate a mixture containing acetone, acetamide and 1,2-dichloroethane using a high-performance liquid chromatography (HPLC) technique. The instrument was equipped with a 40-cm column packed with cyano propyl bonded to the siloxane backbone material. i) List THREE (3) examples of mobile phase solvent/solvent systems that can be used with the column above. ii) Determine the sequence in which the three analytes eluted from the column and explain the underlying principle applied. iii) Sketch the chromatogram that would be produced if the column was replaced with a column filled with C18 hydrocarbon bound to the siloxane backbone material and acetonitrile was employed as the mobile phase. Label the peaks observed.the efficiency of a chromatographic column improves the measure thata.increases the height of the theoretical dishesb.decreases the number of theoretical platesc.platform height is reducedd.None of the above Please explain it Provide clear handwriting and clear imageArrange in INCREASING Rf if the mixture underwent paper chromatography with the same solvent system. Explain how you get the answer I. 2-chloropentaneII. pentanoic acidIII. cyclopentaneIV. pentanal a. II = IV < III < I b.II < IV < I < III c.I < II < III < IV d.III < I < IV < II
