How will the following affect resolution during microscopy? i. Closing or opening the diaphragm ii. Raising or lowering the condenser ii. Increasing or reducing the light intensity
Microscopic examination
The analysis of minute organisms, cellular organization of any biological structure, and composition of body fluids with the help of a microscope is known as microscopic examination. The magnification of specimens or samples under study helps in attaining a clearer picture of it.
Gram Staining
Named after Hans Christian Gram, a Danish bacteriologist, Gram stain is one of the most powerful staining techniques within microbiology. This technique was introduced in 1882 to identify pneumonia-causing organisms. The Gram staining technique uses crystal violet or methylene blue as primary staining colors to distinguish gram-positive from gram-negative organisms. Under a microscope, the gram-positive organisms appear purple-brown, retaining the primary color. Gram-negative organisms appear pink or red as they do not acquire the color of the primary stain.
1. How will the following affect resolution during microscopy?
i. Closing or opening the diaphragm
ii. Raising or lowering the condenser
ii. Increasing or reducing the light intensity
2. During the calibration of an ocular micrometer, two (2) stage micrometer divisions were observed to line up with ten (10). If the graduation interval on the stage micrometer is 0.01mm, calculate:
i. The calibrated value of the ocular micrometer in um.
ii. The size of Pseudomonas aeruginosa which has occupied 5 ocular divisions.
3. What are the methods of staining?
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