I have this practise question and hav no idea where to start. Please help. You are supplied with the following: NaCl (Mr = 58.443 g/mol) 2.5 M Tris-Cl, pH 8 oplossing / solution (1 Litre) EDTA, sodium salt (Mr = 380.2 g/mol) 10 % Sodium dodecyl sulfate solution Proteinase K solution (50 mg dissolved in 1 ml ddH2O) You need a digestion buffer consisting of the following: 15 mM NaCl 75 mM Tris-Cl, pH 8 16 mM EDTA, pH 8 0.8 % Sodium dodecyl sulfate 0.75 mg/ml proteinase K How will you prepare 500ml of digestion buffer? Show all the steps.
Proteins
We generally tend to think of proteins only from a dietary lens, as a component of what we eat. However, they are among the most important and abundant organic macromolecules in the human body, with diverse structures and functions. Every cell contains thousands and thousands of proteins, each with specific functions. Some help in the formation of cellular membrane or walls, some help the cell to move, others act as messages or signals and flow seamlessly from one cell to another, carrying information.
Protein Expression
The method by which living organisms synthesize proteins and further modify and regulate them is called protein expression. Protein expression plays a significant role in several types of research and is highly utilized in molecular biology, biochemistry, and protein research laboratories.
I have this practise question and hav no idea where to start. Please help.
You are supplied with the following:
NaCl (Mr = 58.443 g/mol)
2.5 M Tris-Cl, pH 8 oplossing / solution (1 Litre)
EDTA, sodium salt (Mr = 380.2 g/mol)
10 % Sodium dodecyl sulfate solution
Proteinase K solution (50 mg dissolved in 1 ml ddH2O)
You need a digestion buffer consisting of the following:
15 mM NaCl
75 mM Tris-Cl, pH 8
16 mM EDTA, pH 8
0.8 % Sodium dodecyl sulfate
0.75 mg/ml proteinase K
How will you prepare 500ml of digestion buffer? Show all the steps.
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