Isocitrate labeled with 14C at positions 2 and 5 was isolated from cultured liver cells administered [methyl-14C pyruvate] and enough malonate to completely inhibit succinate dehydrogenase

Q: what is the key enzyme of the pentose phosphate pathway also serving as the dirst enzyme of the…

A: The initial metabolite of the pentose phosphate pathway is glucose-6-phosphate, 2 NADP+, and H2O.…

Q: Since the enzyme is specific for the b anomer, how can the results be interpreted as total glucose?

A: The enzyme glucose oxidase measures the blood glucose level. The enzyme is specific for D-glucose…

Q: The end products of tryptophan degradation are indole and pyruvicacid. Why do we test for the…

A: Indole test is a qualitative biochemical test conducted on bacterial species. It is used to detect…

Q: - [methyl-1“C]Pyruvate was administered to isolated liver cells in the presence of sufficient…

A: Introduction: Pyruvate is the end product of glycolytic pathway. The structure of pyruvate is:

Q: The substrate which was used to investigate the enzymatic activity of wheat bran phosphatase was…

A: Base was added so that pH will become high as it can show the pink color. If pH will not be high…

Q: If [1-14C] Ribose-5-phosphate is incubated with a mixture of purified transketolase-…

A: a. The statement is true. Pentose phosphate pathway is also called hexose monophosphate shunt that…

Q: Which of the following does not correctly describe the isozymes of lactate dehydrogenase (LDH) in…

A: Lactate dehydrogenase (LDH) is an enzyme which catalyzes the conversion of lactate to pyruvate along…

Q: 2-Bromopalmitoyl-CoA inhibits the oxidation of palmitoyl-CoA by isolated mitochondria but has no…

A: Fatty acid oxidation or β- oxidation is the process in which there is the removal of two carbons in…

Q: What are the three major pathways that eventually become entry points of molecules into the Krebs…

A: We'll answer the first question since the exact one wasn't specified. please submit a new question…

Q: 2-carboxyarabinitol-1,5-bisphosphate (CABP) is an inhibitor of the enzyme rubisco. Among A, B, C, D,…

A: The first main step of fixation of carbon is mobilized by the enzyme rubisco. This process helps the…

Q: [methyl-14C]Pyruvate was administered to isolated liver cells in the presence of sufficient malonate…

A: Succinate dehydrogenase or respiratory complex II is an enzyme complex, found in many bacterial…

Q: List the components of the enzyme complex involved in the transition step   and  Simply describe…

A: Components of the enzyme complex :-

Q: Consider the metabolic pathway show below that converts substrate A to B with the enzyme A-ase, B to…

A: For regulation of A-ase: Feedback inhibition refers to the inhibition of the enzymes's activity by…

Q: Which complex contains Succinate dehydrogenase and what are the roles of thisenzyme?

A: Succinate dehydrogenase is a part of the cellular biochemical machinery for breaking down sugars,…

Q: One of the regulators of the TCA cycle is succinyl CoA.  Discuss the rationale for this molecule to…

A: The TCA or Tricarboxylic acid cycle, also called as the citric acid cycle, is a series of reactions…

Q: Some archaebacteria produce an enzyme with two active sites: one catalyzes the dephosphorylation of…

A: Archaebacteria contains an enzyme Fructose-1,6-bisphosphate aldolase which is a bifunctional enzyme…

Q: One of the regulators of the TCA cycle is succinyl CoA.  Discuss the rationale for this molecule to…

A: TCA cycle is also known as the Krebs cycle. This cycle occurs in the mitochondrial matrix. In this…

Q: All the following amino acids produce an intermediate of the TCA cycle except Isoleucine Leucine…

A: TCA cycle or tricarboxylic acid cycle also known as Krebs cycle operates in the mitochondria

Q: Which of the following statements is/are TRUE for the Krebs' cycle? Reaction 1: condensation of…

A: Krebs cycle is the typical process for fully oxidizing proteins, lipids, and carbohydrates as they…

Q: What is the substrate that is not produced during the non-oxidative phase of the pentose phosphate…

A: Asked : The substrate not produced during the non-oxidative phase of the pentose phosphate pathway

Q: Using information we have seen about lactate dehydrogenase, how could you clone and express human…

A: DNA or Gene cloning is the process of making multiple, identical copies of the gene of interest.…

Q: Name the three amino acids (AA) intermediate in the urea cycle.          2. What are the four…

A: Urea cycle is a series of biological reactions in which ammonia is converted into urea. Urea cycle…

Q: 14C-Labeled glyceraldehyde 3-phosphate was added to a yeast extract. After a short time, fructose…

A: Glyceraldehyde 3-phosphate (G3P) is also known as triose phosphate or 3-phosphoglyceraldehyde. It is…

Q: Why do we use the artificial substrate p nitrophenyl glucopyranoside instead of the natural…

A: Enzymes are typically proteins that act as catalysts, speeding up chemical reactions that would take…

Q: What are the reasons for glucuronide conjugation being a major phase II metabolic route?

A: Biotransformation is a chemical modification of biomolecules like nutrients, amino acids, toxins and…

Q: Which of the following is an important end product of disassembly of fatty acids? answer choices…

A: Fatty acids are defined as the carboxylic acids having long aliphatic chains that can be either…

Q: both complex I and complex II send electrons to cytochrome c, how many cytochrome c molecules will…

A: Electrons from Nadh pass through a flavoprotein to a series of iron sulphur proteins in complex I to…

Q: [1-14C]Ribose-5-phosphate is incubated with a mixture of purified transketolase, transaldolase,…

A: Ribose-5-phosphate gets converted to seduheptulose-7-phosphate by transketolase and…

Q: Normal cells die in a nutrient medium containing thymidine and methotrexate, whereas mutant cells…

A: Methotrexate is a chemotherapy agent which is used to treat cancer, autoimmune diseases, ectopic…

Q: What enzyme can catalyze the committed step of the pentose phosphate pathway? Please choose one…

A: The pentose phosphate pathway is also known as hexose monophosphate (HMP) shunt or phospho gluconate…

Q: [methyl-14C]Pyruvate was administered to isolated liver cells in the presence of sufficient malonate…

A: When the radioactive pyruvate at methyl carbon (carbon number 3) would be administered to the liver…

Q: Are there any specific enzymes Mycobacterium tuberculosis has for identification (examples include…

A: Mycobacterium tuberculosis, the bacteria that causes tuberculosis (TB), is a highly effective…

Q: What are the structural elements that are required for proper function in yeast alcohol…

A: During fermentation of glucose yeast alcohol dehydrogenase reduces acetaldehyde to ethanol. Glucose…

Q: Which of the following Krebs cycle enzymes generates a molecule of FADH2 from FAD? a-ketoglutarate…

A: The citric acid cycle or the Krebs cycle is a series of chemical reactions used by all aerobic…

Q: . Using the principles described in the text regarding pyridoxal phos- phate mechanisms, propose a…

A: Introduction- Serine hydroxy methyltransferase (SHMT) is known as a pyridoxal phosphate-dependent…

Q: Which of the following enzymes below is/are involved in the control of Krebs' cycle? citrate…

A: Kreb cycle also known as TCA cycle. It occur in matrix of mitochondria in eukaryotes and cytosol in…

Q: A molecule -X inhibits oxidation of stearoyl-CoA by isolated mitochondria but has no effect on…

A: Sterol-CoA desaturate is referred to as the key enzyme that is involved in the process of…

Q: As stated in the text, mammalian cells can become resistant to the lethal action of methotrexate by…

A: Methotrexate : It is a chemotherapy agent and immune system suppressant. It is used to treat cancer,…

Q: Is trimethoprim a mechanism-based inhibitor of bacterial dihydrofolate reductase?

A: DHFR the dihydrofolate reductase it is an enzyme that helps in purine synthesis.

Q: which substrate molecule serves as the phosphate donor during substrate-level phosphorylation in…

A: Introduction: Substrate Level Phosphorylation : Substrate-level Phosphorylation Is A Metabolic…

Q: If the pyruvate is labelled with 14C at its carbonyl carbon, how many rounds of the TCA cycle are…

A: Glycolysis is the primary biochemical pathway of cellular respiration that involves the conversion…

Q: What is the reduced product of complex IIII/cytochrome oxidase????

A: Cytochrome c oxidase (CcO) is a respiratory energy-transducing enzyme. It catalyzes electron…

Q: The natural sulfur cycle involves many sulfur- reducing and sulfur-oxidizing bacteria. For example,…

A: Sulphur cycle describes the flow of Sulfur from inorganic component to organic component of the…

Q: The rate limiting and committed step of the pentose phosphate pathway is catalyzed by which of the…

A: The pentose phosphate pathway (PPP) is a different process for metabolizing glucose. It is divided…

Q: What are the main products of the pentose phosphate pathway? (both branches, select all that apply)…

A: Metabolism is a series of chemical reactions that occur within the living body for the production of…

Q: Why is it important that the Embden-Meyerhof, Pentose Phosphate, and Entner-Doudoroff pathways each…

A: Cellular respiration takes place in the cells of the plants and animals that convert the chemical…

Metabolic pathways

Metabolism is defined as the group of life-supporting, enzyme-mediated chemical reactions in organisms. A metabolic pathway is a set of these chemical reactions that involve anabolism (synthesis of complex biomolecules) and catabolism (breakdown of biomolecules into small molecules) that occur inside a cell. Metabolites are the reacting substances, products, and intermediate substances of an enzymatic process that are altered by a series of chemical events catalyzed by enzymes.