It's important to mark the solvent front with a pencil after the plate is finished being developed in order to calculate an Rf. Group of answer choices True False
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- Solvent #1 is a 50:50 mixture of hexane and ethyl acetate and solvent #2 is 25% hexane. Which solvent mixture is more polar: the 50:50 mixture or the 25:75 mixture? Explain briefly What would happen to the Rf of the spots in solvent #2 compared to solvent #1? Why?What is the Rf value for a compound if it moves 3.0 cm from the origin when the initial spot is developed and the solvent front is 12.0 cm from the origin?Create a detailed flow sheet for the following experiment.
- Recrystallized 2-naphthol compared to literature values • Format the table and the descriptive paragraphs. Full comparisons of peaks are to be made with reference to the SDBS database for all three compounds.Q1: Give one lysis buffer that is commonly used for western blotting experiments and include its components Q3: To make sure that you used a similar amount of samples, what important step should be done before proceeding the electrophoresis stage? Q4. Why is it necessary to store the prepared lysates in a very low temperature?Can someone help me figure out which analytical method would be the most effective for this probelm? I don't think B (melting pt) and C (infrared spectroscopy= functional groups) are the most effective.
- Propose an extraction flowchart capable of separating this compound into its pure form2 Perform theoretical IR and NMR analysis on paracetamol. Summarize your ideas in 2 charts.Recrystallized Benzoic acid compare to literature values • Format the table and the descriptive paragraphs. Full comparisons of peaks are to be made with reference to the SDBS database for all three compounds.
- 2-naphthol IR Tables that contains the important peaks for the experimentalIn your own words, why is it false that the selection of the functional unit is primarily driven by the LCA practitioner’s preferences and does not significantly affect the LCA results?Compare acetanilide, which was in the recrystallization lab and acetaminophen (2) shown below. Which compound do you predict would have a larger Rf value? Briefly explain in less than 10 words.