1 Esters can be used as solvents in chromatography. The following shows a chromatogram of plant acids. solvent front the cross represents the centre of the spot baseline sample 1 sample An ester was used as the solvent and the chromatogram was sprayed with bromothymol blue. (1) Suggest why it was necessary to spray the chromatogram. (ii) Explain what is meant by the Rf value of a sample. (iii) Calculate the Rf values of the two samples and use the data in the table to identify the plant acids.

EBK A SMALL SCALE APPROACH TO ORGANIC L
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Chapter91: Gas Chromatography
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1 Esters can be used as solvents in chromatography. The following shows a chromatogram of plant acids.
solvent front
the cross represents
the centre of the spot
baseline
sample 1
sample
An ester was used as the solvent and the chromatogram was sprayed with bromothymol blue.
(1) Suggest why it was necessary to spray the chromatogram.
(ii) Explain what is meant by the Rf value of a sample.
(iii) Calculate the Rf values of the two samples and use the data in the table to identify the plant acids.
Transcribed Image Text:1 Esters can be used as solvents in chromatography. The following shows a chromatogram of plant acids. solvent front the cross represents the centre of the spot baseline sample 1 sample An ester was used as the solvent and the chromatogram was sprayed with bromothymol blue. (1) Suggest why it was necessary to spray the chromatogram. (ii) Explain what is meant by the Rf value of a sample. (iii) Calculate the Rf values of the two samples and use the data in the table to identify the plant acids.
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