Match each characteristic to the proper bacterial fertility factor type.
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- Formation of a(n) ___ allows some soil bacteria to survive adverse conditions. a. pilus b. nucleoid c. endospore d. plasmidThe addition of DNase to a mixture of donor and recipientcells will prevent gene transfer viaa) DNA transformation.b) chromosome transfer by conjugation.c) plasmid transfer by conjugation.d) generalized transduction.Because bacteria are able to replicate at high rates, they aresubjected to mistakes or mutations being made in their nucleo-tide sequence. For example, the nucleotide sequence in a genecoding for a protein might be AATTGGCCA but because ofmutation might become GGTTGGCCA. How may such a mu-tation become beneficial to the bacterium? How may it becomeharmful?
- 1. Write if the statement is true and modify the statement with the correct answer if its false a.White colonies represent those bacteria that do not express the genes for beta-galactosidase because restriction enzymes have cut the gene and ligase have not repaired the break b.Antibiotic resistance genes in bacterial transformation are used as marker genes because of ease of detection of transformants. c. Bacterial transformation simply means the insertion of a recombinant plasmid into a bacterial cell.Researchers studying the bacterium Escherichia coli split a population of the bacteria into two samples.Sample 1 was transformed with a plasmid containing a gene that makes the bacteria resistant to theantibiotic kanamycin. Sample 2 was transformed with a plasmid lacking the antibiotic resistance gene.A portion of each sample was then added to growth plates containing just nutrients or growth platescontaining nutrients and kanamycin.After being allowed to grow for 24 hours at 37 0 C , the number of colonies on each growth plate wascounted (Table 1).Table 1. BACTERIAL GROWTH FOLLOWING TRANSFORMATION WITH OR WITHOUT THEKANAMYCIN RESISTANCE GENEPlace # Transforming plasmidContains KanamycinResistance Gene Growth Media Results 1 No Nutrient Only Too may colonies to count (lawn)2 yes Nutrient Only Too any colonies to count (lawn3 No Nutrient with Antibiotic No colonies observed4 Yes Nutrient with Antibiotic 7 colonies observedWhich of the following claims is best supported by the data in…The plasmid (episome) F factor mediates which of the following? a. Transduction b. Transformation c. Conjugation d. Replication e. Translocation
- Drug-resistance plasmids could be transferred in A. baumannii bywhich of the following mechanisms:a. conjugation pilus d. a and bb. bacteriophages e. a, b, and cc. capture by cell wallResearchers studying the bacterium Escherichia coli split a population of the bacteria into two samples. Sample 1 was transformed with a plasmid containing a gene that makes the bacteria resistant to the antibiotic kanamycin. Sample 2 was transformed with a plasmid lacking the antibiotic resistance gene. A portion of each sample was then added to growth plates containing just nutrients or growth plates containing nutrients and kanamycin. After being allowed to grow for 24hours at 37∘C, the number of colonies on each growth plate was counted (Table 1). Which of the following claims is best supported by the data in Table 1 ? a.More bacteria on plates 1 and 2 were successfully transformed than on any other plate. b.Only the bacteria that were successfully transformed with the kanamycin resistance gene grew on plate 4. c.The transformation procedure killed all the bacteria that were added to plate 3. d.None of…A) A bacterium that has an F plasmid is able to do which of the following? Group of answer choices resist antibiotics survive in very harsh environments such as hypersaline lakes grow a cell wall form conjugation tubes and pass on their DNA to other bacteria reproduce sexually B) horizontal gene transfer refers to bacteria's ability to Group of answer choices make peptidoglycan for their cell walls transfer DNA to offspring, or "daughter" cells transfer DNA only when on horizontal surfaces, such as a table top transfer pieces of DNA to other cells in the population engage in mutualisms with certain species
- The term "Superbug" refers to? A. Certain bacteria strain that can grow in all kinds of unfavorable conditiions B. Certain bacteria strain is more toxic than all the viruses C. Certain bacteria strain that is resistant to all available antibiotics D. Certain bacteria strain that grows faster than any other strainsWhich of the statements is TRUE about conjugation? a) Plasmid DNA is transferred from a donor (F+) cell b) It may only be performed by competent cells c) DNA is always integrated into recipient chromosome d) It may be specialized or generalizedIf a bacteria cell is F+, that cell must be capable of transduction. contain the fertility factor. be capable of transformation. contain antibiotic resistant plasmids.
