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Multiprotein complexes are formed by weak noncovalent interactions between the proteins. Suppose proteins A and B form a heterodimer with a Ka = 10-6 M: [A][B] [AB] AB =A+ B Kg = 10-6M (a) Calculate the fraction of monomers that exist in the heterodimer complex if the total concentration of A + B inside the cell is 5mM. Assume [A]total = [B]total-

Multiprotein complexes are formed by weak noncovalent interactions between the proteins. Suppose proteins A and B form a heterodimer with a Ka = 10-6 M: [A][B] [AB] AB =A+ B Kg = 10-6M (a) Calculate the fraction of monomers that exist in the heterodimer complex if the total concentration of A + B inside the cell is 5mM. Assume [A]total = [B]total-

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
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Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Multiprotein complexes are formed by weak noncovalent interactions between the proteins. Suppose proteins A and B form a heterodimer with a Kg = 10-6 M: [A][B] - 10-6M [AB] AB =A+B Kg= (a) Calculate the fraction of monomers that exist in the heterodimer complex if the total concentration of A + B inside the cell is 5mM. Assume [A]total = [B]total- (b) If the cell is now disrupted in buffer, the cell contents are diluted 5-fold. What fraction of monomers would exist in the complex under these conditions? (C) What does this calculation tell you about detecting multiprotein complexes in cell-free homogenates? Should they be readily detectable? If not, what methods might you use to aid in their detection?
Transcribed Image Text:Multiprotein complexes are formed by weak noncovalent interactions between the proteins. Suppose proteins A and B form a heterodimer with a Kg = 10-6 M: [A][B] - 10-6M [AB] AB =A+B Kg= (a) Calculate the fraction of monomers that exist in the heterodimer complex if the total concentration of A + B inside the cell is 5mM. Assume [A]total = [B]total- (b) If the cell is now disrupted in buffer, the cell contents are diluted 5-fold. What fraction of monomers would exist in the complex under these conditions? (C) What does this calculation tell you about detecting multiprotein complexes in cell-free homogenates? Should they be readily detectable? If not, what methods might you use to aid in their detection?
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