Name Date Lab Report: 14 Bacteriophage Specificity 1. What was the purpose of this exercise? Complete the following table with your observations: Name of the bacterium Lysis (yes / no) 5 lague formation E.COi yes Entechacter averogens Staphliococcus NO aureus How do you know if bacteriophage infected the bacteria? "clear circles' die Plagues, where has lysed the ba cteriom 3. the phage DN A Why didn't the bacteriophage infect all three bacteria? Sequencing 4. What is the explanation for bacteriophage specificity when it comes to infecting specific bacterium? Proten nucle 87 2. 5.
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- PLEASE ANS ASAP 1. When placed into a hypertonic solution, a bacterial cell will: a:die as a result of dessication. b: lyse c: swell d. take in more water than it releases . 2. Which one of the following methods of antimicrobial susceptibility testing requires a dilution series of the antibiotic and can provide a minimum inhibitory concentration? a. Gradient diffusion method b. Agar macrodilution method c. Broth microdilution method d. Disk diffusion method 3. Blood for culture is usually obtained from a vein located in which area? a. Brachial b. Femoral c. Axillary d. Antecubital 4. All clinical specimens submitted to the clinical microbiology laboratory must be: a. all of these b. property and carefully collected. c. properly labeled. d. properly transported to the laboratory 5. Contact precautions are required for patients with a. all of these b. respiratory syncytial virus c. Clostridium difficile c. associated diseases. d. infections caused by multidrug-resistant…Please ASAP. Thanku. An animal blood cell in a beaker of high salt water will become ___________________. Turgid Shriveled (crenated) Plasmolyzed Lysedhttp://vlab.amrita.edu/?sub=3&brch=73&sim=1628&cnt=1 Explain how the Kirby-Bauer method relies on diffusion of antibiotics
- a. Explain whether or not any of the methods in fi gure 2.9 could beused to determine the total number of cells present in a patient’s specimen.b. After performing the streak plate method on a bacterial specimen, theculture was incubated for 48 hours at 37°C. Upon viewing the plate, therewas heavy growth (with no isolated colonies) in the fi rst quadrant, but nogrowth was apparent in the remaining quadrants. Please discuss errors in the procedure that could have produced this result.Hi, I want to ask the question related to antibiotics resistance? The question is the non-existance of solidifying agent may produce a liquid like culture media. Name the method used to determine the Antibiotic Susceptibility test which uses the said media? Can explain answer? Thank you.Please answer fast What is this organism? And what other test could be done to confirm it's identification? 1. Gram stain - positive cocci, chains Hemolysis- gamma BE - positive NaCl - positive Catalase - weakly positive 2. Gram positive cocci, chains NaCl - negative BE - negative Hippurate - positive Hemoylsis - beta Catalase - negative
- serology (elisa) lab: If the sample gave a negative result for the disease-causing agent, does this mean that you do not have the disease? What reasons could there be for a negative result when you actually do have the disease?Amswer the following questions: 1. What other physiological responses may have caused the diphasic growth in E. coli? 2.Describe the methods used by microbiologists in producing synchronous cultures from unsynchronized bacterial culture.Activity 13 Urine Culture Inoculating Urine with a calibrated loop The number of microorganisms per milliliter recovered on urine culture can aid in the differential diagnosis of urinary tract infection (UTI). Plastic or wire inoculating loop available commercially, have been calibrated to deliver a known volume of liquid when handled correctly, thus enabling the microbiologist to estimate numbers of organism in the original specimen based on colony forming unit (CFU) of growth on cultures. PROCEDURE: 1st day 1. Gently swirl the specimen bottle to mix the urine specimen. 2. Label all plated media with name of patients, clinical specimen used and date. Label at the bottom of plates and not on the cover. 3. Obtain a disposable calibrated loop. 4. Dip the loop straight into the urine specimen so that the loop part is completely covered. Withdraw straight out. See fig. 16-18 5. Inoculate blood agar plate (BAP) as shown in fig.16-19 6. Incubate at 35 – 37˚C for 18 – 24 hours. 7. Dispose…
- 1. What is the critical threshold between bacterial infection and simple bacterial contamination? 2. Why is aseptic technique important in urine collection? 3. Give at least 5 bacteria that can cause Urinary Tract Infection.Plaque assay (Bacteriophage Titer) Answer questions 2 & 3 2. Why don't bacteriophages continue spreading over the entire plate until all of the E. coli cells on the plate are lysed? 3. Why is it important that bacterial cells should be in the exponential growth phase for this experiment?1. Give 2 systems used at present for the automatic identification of bacteria. 2. Why aseptic technique important in urine collection for culture? 3. What are the specimen consideration you have to remember in preparing blood culture? 4. Being a medical technology student, how can you contribute to avoid water pollution and degradation of natural resources?