5. In this serial dilution process, WHICH PLATE will the microbiologist chose to count colonies for the greatest accuracy? 1 ml Original Culture 1 ml 1 ml 1 ml 1 ml mcuture whoer 0rion 9 ml dilution 1/130 1/10.000 /00000 V1000 tubes 10 20 1 ml 1 ml 1 ml 1 mL mL Growth of colonies on pour plates after incubation Toomany >50d to count 71 colonies 1500 O 71 O NONE OF THE ABOVE
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- 1. Which of the following is NOT true of spread plating method? A. the maximum inoculum allowed is 1.0 ml B. spreading of the inoculum is done using a disinfected glass L-rod C. A pre-solidified medium is required prior to inoculation of the sample. D. A and B 2. Plates inoculated with bacteria are inverted during incubation to: A. prevent contamination B. limit the colony growth C. A and B D. A and C E. All of the above F. None of the above1. Explain why the following steps are essential during sub-culturing: a. Flaming the inoculating instrument prior to and after each inoculation b. Holding the test tube caps in the hand as illustrated in Figure 1. c. Cooling the inoculating instrument prior to obtaining the inoculum d. Flaming the neck of the tubes immediately after uncapping and before recapping 2. Describe the purposes of the sub-culture procedure. 3. Explain why a straight inoculating needle is used to inoculate an agar deep tube. 4. What is the indication of bacterial growth in each of the media? (nutrient broth, nutrient agar slant, nutrient agar deep? 5. Enumerate the Good Microbiological Practices encountered in the activity. 6. Upon observation of the nutrient agar slant culture, you strongly suspect that the culture is contaminated. Outline the method you would follow to ascertain whether your suspicion is justified.1. Calculate the CFU/mL of the original culture for the countable plate as shown in the diagram. b) How many colonies would you expect if you plated out 0.1 mL from Tube C. c) How many colonies would you expect if you plated out 1.0 mL from Tube C.
- An outbreak of Methicillin-resistant Staphylococcus aureus (MRSA) in the surgical ward at the hospital forces the hospital administrator to cancel and postpone all non-essential surgeries until the source of infection has been located and for measure put into place. Bacterial samples obtained from swab of wad staff equipment and surfaces arr brought to the lab for analysis. 1. What testing method will you employ to determine the level of exposure? 2 How would a positive test present? 3. How would a negative test present? 4. What recommendations would you make in your report?1. What is one advantage of utilizing the pour plate technique over the streak plate technique ? 2. Why must the agar pours be cooled to 45C before use in the pour plate technique? 3. Explain the consequences if a group removed all the agar pours from the water bath at one time and allowed them to sit on the bench for several minutes before using them. 4. Why can the agar pour tubes be rinsed in the sink after the agar is transferred to the Petri plate ? Could you rinse the tubes if the bacteria had been pipetted into the agar pour tubes rather than in the plates? Explain. 5. What would be the result if a student dipped his / her loop in the stock culture during inoculations of each quadrant ? Explain . part B 1. The introduction stated that microbes are mechanically separated or diluted over the surface of the medium . How is this accomplished ? 2. Go to https://commons.wikimedia.org/wiki/File:MacConkey_agar_with_LF_and_LF_colonies . . Which side of the plate (left or right)…PLEASE ANS ASAP 7. Typhoid fever is caused by a species of: a . Escherichia b. Campylobacter c. Shigella. d. Salmonella 8. At the end of the Gram -staining procedure , Gram -positive bacteria will be: a. blue to purple b . pink to red c. orange. d. green 9. Which one of the following types of culture media is selective and differential? a. Phenylethyl alcohol agar b . Blood agar c. Thayer-Martin agar d. MacConkey agar 10. Which one of the following methods of antimicrobial susceptibility testing combines some of the properties of two other methods and involves adding a plastic strip to the agar? a. Gradient diffusion method b. Agar macrodilution method c. Disk diffusion method d. Broth microdilution method 11. Most ATP molecules are produced during which phase of aerobia respiration? a. Fermentation b. Krebs cycle c. Glycolysis d. Electron transport chain 12. The goal of disinfection is to eliminate ____ whereas the goal of sterile technique is to exclude ____ a.…
- 1. Use the words presented in below to create a concept map illustrating the relationships among these key terms – refer to “Five I’s” by Cowan and Smith (2018) to be guided. inoculation identification biochemical tests streak plate isolation medium subculturing mixed culture incubation multiplication source of microbes pure culture inspection staining transport medium wire loop 2. What is the importance of employing aseptic techniques? Give an example of a situation in the laboratory that might happen when this method is not practiced.2. You use tubes to test aerotolerance of bacteria. From your samples you have 3 results: A. Bacteria growing on the surface. B. Bacteria growing throughout the tube, the agar shows cracks. C. Bacteria growing about 5 mm below the surface. Please interpret each bacterial result. (Give the bacteria an oxygen classification, explain what classification means and interpret the cracks in the agar.)1. The technique used in bacteria for endospore staining can also be used to observe fungal spores. Select one: True False 2. Which of the following statements is/are TRUE? a. Agar does not provide nutrients to microorganisms. b. Peptone, pork extracts, and yeast extracts provide nutrients to microbes. c. Tap water must be used for preparing culture media. d. A & B are correct. e. B & C are correct Clear my choice 3. Motility of microorganisms is BEST observed with a a. Flagellar stain b. Hanging drop preparation c. Solid medium d. Negative stain e. Streak plate Clear my choice
- 8. The time of the incubation for the serial dilution experiment should be ___ hrs.: a) 12 b) 24 c) 48 d) one week 9. The autoclave temperature must be at least ___ : a) 100 oC b) 100 oF c) 121.6 oC d) 121.6 oF 10. The use of the autoclave ensures: a) the death of all bacteria b) the death of all bacterial spores c) all of the above d) none of the above.1) would you describe the contents of the soil-inoculated broth as being a “pure culture”? Why or why not? 2) How did the uninoculated broth differ in appearance from the broths inoculated with E. Coli and M. Luteus? And then how could you tell if a supposedly sterile, uninoculated broth was contaminated? Please explain in detail and highlight the important parts cuz I am confused and need help! ThanksThe main of objective of using 13-Line streaking method is to get: A-Contaminated cultures B-None of the above C-Overlapped colonies D-Separated colonies