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1. What is the critical threshold between bacterial infection and simple bacterial contamination?
2. Why is aseptic technique important in urine collection?
3. Give at least 5 bacteria that can cause Urinary Tract Infection.
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- 2. Mechanical disinfection is typically more effective than manual techniques.1. Give 2 systems used at present for the automatic identification of bacteria. 2. Why aseptic technique important in urine collection for culture? 3. What are the specimen consideration you have to remember in preparing blood culture? 4. Being a medical technology student, how can you contribute to avoid water pollution and degradation of natural resources?2. What microorganisms other than coliforms are likely to give positive presumptive test?
- 1. Explain why the following steps are essential during sub-culturing: a. Flaming the inoculating instrument prior to and after each inoculation b. Holding the test tube caps in the hand as illustrated in Figure 1. c. Cooling the inoculating instrument prior to obtaining the inoculum d. Flaming the neck of the tubes immediately after uncapping and before recapping 2. Describe the purposes of the sub-culture procedure. 3. Explain why a straight inoculating needle is used to inoculate an agar deep tube. 4. What is the indication of bacterial growth in each of the media? (nutrient broth, nutrient agar slant, nutrient agar deep? 5. Enumerate the Good Microbiological Practices encountered in the activity. 6. Upon observation of the nutrient agar slant culture, you strongly suspect that the culture is contaminated. Outline the method you would follow to ascertain whether your suspicion is justified.1.Would a syringe filter with a pore size of 0.22 micrometers be able to completely remove all microorganisms including viruses? Explain why or why not? 2. Under what conditions is sterilization by filtration preferred over sterilization by heat? (short answer)1. What is the key ingredient of hand sanitizers?  include a image of the chemical structure of the ingredient and explain how it works 2. What did Ignacio Semmelweis discover? Describe the situation and what he recommended to the medical community 
- An outbreak of Methicillin-resistant Staphylococcus aureus (MRSA) in the surgical ward at the hospital forces the hospital administrator to cancel and postpone all non-essential surgeries until the source of infection has been located and for measure put into place. Bacterial samples obtained from swab of wad staff equipment and surfaces arr brought to the lab for analysis. 1. What testing method will you employ to determine the level of exposure? 2 How would a positive test present? 3. How would a negative test present? 4. What recommendations would you make in your report?1. How significant is cellulose tape perianal swab to the control of pinworm infection in the community? 2. What is the significance of properly labeling collected specimen?1. Which of the following is NOT true of spread plating method? A. the maximum inoculum allowed is 1.0 ml B. spreading of the inoculum is done using a disinfected glass L-rod C. A pre-solidified medium is required prior to inoculation of the sample. D. A and B 2. Plates inoculated with bacteria are inverted during incubation to: A. prevent contamination B. limit the colony growth C. A and B D. A and C E. All of the above F. None of the above
- 1. Define and Describe aseptic technique in blood collection. 2. Explain the action of SPS as anticoagulant for blood culture.4. What other test must be done to confirm the presence of E. coli?Hi, I want to ask the question related to antibiotics resistance? The question is the non-existance of solidifying agent may produce a liquid like culture media. Name the method used to determine the Antibiotic Susceptibility test which uses the said media? Can explain answer? Thank you.
