What is the CFU per mL when a 0.7mL stock suspension containing 2.1 x 10^9 CFU per ml was diluted to 4.3mL 0.1M K2HPO4-KH2PO4 buffer
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1. What is the CFU per mL when a 0.7mL stock suspension containing 2.1 x 10^9 CFU per ml was diluted to 4.3mL 0.1M K2HPO4-KH2PO4 buffer?
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- 1. Using the equation of the line, cell density can be measured, EXCEPT? * A. When the absorbance reading is 0.3. B. when the absorbance reading is 0.1 C. When the absorbance reading is 0.7 D. All are correct 2. These microscopes produce an image through refractive indices except * A. brightfield microscope B. transmission electron microscope. C. differential interference contrast microscope. D. phase-contrast microscope.3. Explain the following terms and what are its corresponding requirements for Raman applications: a. Confocal microscope b. Numerical aperture c. Infinity correction d. F-number1. In microscopy, what could be the possible reason why we cannot completely resolve the specimen under the Oil Immersion Objective (OIO) when, in fact, it was taught that the OIO has a resolving poewer? 2. When using the OIO, we can only focus on one group or parts at a time. (that is, the other parts are blurred) Whys is it so?
- You have a microscope equipped with 10 x oculars and 4 objectives, which exhibit the following characteristics (M, NA): A=(4 x/0.13); B=(20 x/0.4); C=(20 x/0.7); D=(40 x/0.65) Tick the right answers: o Objectives B and C are equivalent. o Objective D will allow more detailed observation than objective C. o To see small structures, you should choose objective D rather than C. o Objective D has a lower resolution than objective C. o To observe large objects, it is better to use objective B rather than objective C1. a) What are Romanowsky stains? Name four of these. b: What are the components of a Romanowsky stain?b: What is the optimum pH for staining with Giemsa. c: Name two conditions which will affect the quality of your staining. d: What is a panoptic stain. Give one example. e: Name two blood parasites that can be demonstrated using the Giemsa stain.In confocal microscopy, what is the theoretical resolving power of the objective used (63X, NA 1.3, oil immersion noil=1.52)? Clearly show your calculations.
- 1. How is the letter “e” on the slide oriented when you see it with the naked eye as you mount it on the stage (i.e., is it right side up or upside down)? 2. How is the letter “e” on the slide oriented when you see it under low or high power magnification? 3. What effect, if any, does the compound light microscope have on the orientation of the image?1. What are the different hoods in the lab and why are they important? 2. How does the light compound microscope magnify an object? Outline the process.FOV measured at 100x total magnification is 400 micrometers. What is the FOV at 40X total magnification? 2. If FOV is 5 mm and the number of cells in FOV is estimated at 35, what is the cell size in mm? In micrometers?
- Answer the following questions: 1. Give the general steps in the use of a spectrophotometer. 2. Define the following: a. ) Optical Density b.) Transmittance c.) Beer's Law1. You're using a microscope with 10x magnification ocular lenses and a 25x magnification objective lens. What is the microscope's overall magnification at this setting? 2. Is it correct that immersion oil improves image magnification and resolution?1. Differentiate the compound light microscope from the dissecting microscope in terms of: *image produced *magnification *type of specimen examined 2. Write down the principles of the following: *electron microscopy *scanning tunneling microscopy *atomic force microscopy
