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- A sample is diluted by a factor of 10 five times. The 10^-3 dilution has 272 colonies on it. Assuming you plated the same volume on each plate, how many colonies would you expect to find on the 10^-4 plate? Include units and use OCD formula.You have performed a serial dilution of an unknown sample and counted 73 CFU on a countable plate that was marked at 10^-4 dilution and you used 0.1 mL to inoculate the plate. What is the population of the original sample?Mary Jo prepared a series of diluted samples by taking 1 mL of sample and adding it to 4 mLs of diluent. She then diluted the sample further by adding 1 mL of diluted sample to 19 mLs of diluent. What is the final dilution factor ?
- You discover that the plate you selected had only been inoculated with 0.1 ML of the dilution instead of one ML. Using the count data you required recalculate the number of CFU's in the original sampleA plate has 72 colonies with a total dilution factor of 10-7, 0.1 ml was pipetted onto the plate, what was the original CFU/ml concentration of that sample?A laboratory technician performed a series of 10 fold serial dilutions shown below. he plated 0.1 mL from each dilution tube onto NA and got the follow numbers per plate dilution 1:10 1:10 1:100 1:1000 1:10000 1:100000 colony count >1000 >1000 808 303 38 3 calculate the concentration of bacteria in the undiluted stock (in CFU/mL)
- You are testing unpasteurized milk for the presence of bacterial contamination. Starting from the undiluted milk, you do serial dilutions, and plate 0.5 ml of 4th dilution on agar. If you counted 78 CFUs from the 4th plate, how many bacteria/ml do you expect? (show solution) Do you think standard plate counts are very accurate? Why or why not?Please explain Now that the laboratory scientist has a 1:10 dilution, what dilution should he make next to create a 1:100 dilution? Choose best letter answer and give a brief explanation. A. 1:2 B. 1:5 C. 1:10 D. 1:100In this experiment, a culture was serially diluted to the concentrations below. Each plate was plated with 0.25mL of the dilution. Using the most diluted plate, what is the correct concentration of the original culture? A) 2.0 X 10^-3 cells/mL B) 2.0 X 10^5 cells/mL C) 4.0 X 10^5 cells/mL D) 5.0 X 10^4 cells/mL
- Describe how you would make 10 ml of a 1:500 dilution of bacteria by performing TWO SEPARATE DILUTIONS in series? Consider the accuracy of the pipetting so that you don’t make a single dilution that is too large.Without water for decontamination, you are forced to improvise. What supplies would you grab? Large trash bags, plastic shower caps, scrubs, permanent markers, foley cath, aspirin, ace bandages? Select all that apply. Explain all point deeply and type the answer.You begin with an overnight culture of approximately 108 organisms/ml and need a concentration of 103 bacteria/ml in order to get a countable spread plate. Describe how you would dilute your sample.