PO4- LOH Incorporation of a deoxynucleotide (DNTP) elongates the chain. A 5- 3'OH subsequent nucleotide can 5' be added to the 3'OH. 3' Incorporation of a dideoxynucleotide (ddNTP); 5- 3'OH 3' the ddNTP lacks a 3'OH. 5' Chain elongation is terminated. No additional nucleotides can be added PO4. HO- 5- 3' 5' due to the lack of a 3'OH. FIGURE 9.11 Chain Termination by a Dideoxynucleotide Once a dideoxynucleotide is incorporated into a growing strand, additional nucleotides cannot be added.

Human Heredity: Principles and Issues (MindTap Course List)
11th Edition
ISBN:9781305251052
Author:Michael Cummings
Publisher:Michael Cummings
Chapter8: The Structure, Replication, And Chromosomal Organization Of Dna
Section: Chapter Questions
Problem 15QP: Using Figures 8.7 and 8.9 as a guide, draw a dinucleotide composed of C and A. Next to this, draw...
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How is a dideoxynucleotide different from a deoxynucleotide?

PO4-
LOH Incorporation of a
deoxynucleotide (DNTP)
elongates the chain. A
5-
3'OH
subsequent nucleotide can
5'
be added to the 3'OH.
3'
Incorporation of a
dideoxynucleotide (ddNTP);
5-
3'OH
3'
the ddNTP lacks a 3'OH.
5'
Chain elongation is
terminated. No additional
nucleotides can be added
PO4.
HO-
5-
3'
5' due to the lack of a 3'OH.
FIGURE 9.11 Chain Termination by a Dideoxynucleotide Once
a dideoxynucleotide is incorporated into a growing strand, additional
nucleotides cannot be added.
Transcribed Image Text:PO4- LOH Incorporation of a deoxynucleotide (DNTP) elongates the chain. A 5- 3'OH subsequent nucleotide can 5' be added to the 3'OH. 3' Incorporation of a dideoxynucleotide (ddNTP); 5- 3'OH 3' the ddNTP lacks a 3'OH. 5' Chain elongation is terminated. No additional nucleotides can be added PO4. HO- 5- 3' 5' due to the lack of a 3'OH. FIGURE 9.11 Chain Termination by a Dideoxynucleotide Once a dideoxynucleotide is incorporated into a growing strand, additional nucleotides cannot be added.
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