Polar solutes were separated by HPLC (not HILIC) with a bonded phase containing polar diol substituents [—CH(OH)CH2OH]. How would retention times be affected if eluent were changed from 40 vol% to 60 vol% acetonitrile in water? Acetonitrile (CH3CqN) is less polar than water.
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Polar solutes were separated by HPLC (not HILIC) with a bonded phase containing polar
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- 2-pentanone has a retention index of 987 on a poly(ethylene glycol) column (also called Carbowax). (a) Between which two straight-chain hydrocarbons is 2-pentanone eluted? (b) An unretained solute is eluted from a certain column in 1.80 min. Decane (C10H22) is eluted in 15.63 min and undecane (C11H24) is eluted in 17.22 min. What is the retention time of a compound whose retention index is 1 050?An unretained solute is eluted from a GC column in 1.80 min. Decane (C10H22) is eluted in 15.63 min and undecan (C11H24) is eluted in 17.22 min. What is the retention time (report to 2 decimal places, in minutes) of a compound whose retention index is 1050?A 25 g of butyric acid was extracted with 400 mL ether from 400 mL water (Kd=3.0 at 25 °C) Using single-step extraction, what is the amount of butyric acid in water layer? What is the total amount of butyric acid extracted using two successive extractions? Calculate the % efficiency of two-step extraction over single
- In a reverse-phase separation, a solute was found to have a retention time of 19.6 min, while an unretained peak had a retention time of 1.45 min when the mobile phase was 20/80 v/v% methanol/water. (b) Calculate the k’ for the solute. (c) Calculate the new k’ if the mobile phase is changed to 60/40 methanol/water assuming identical void volume. (d) Calculate a solvent composition that would bring k’ from initial value to a value of 1.20 using tetrahydrofuran/water mobile phase rather than 20/80 v/v % methanol/water.4g 5 g 2 g 100 g 49." R Lactic acid Salicylic acid Trichloroacetic acid Flexible collodion qs ad Sig: wart remover. Use as directed. (a) Flexible collodion contains 20% w/w camphor and 30% w/w castor oil. How many grams of each would be contained in 30 g of the mixture? (b) The specific gravity of castor oil is 0.955. How many milliliters of the oil is contained in 30 g of the mixture? (c) If the specific gravity of the mixture is 0.781, what are the percent w/v con- centrations of lactic acid, salicylic acid, and trichloroacetic acid in the mixture?4 litres of water obtained from a borewell in Vellore contains FeSO4 = 30.4 mg; CaSO4 = 13.6 mg; MgCl2 = 38 mg; Ca(HCO3)2 = 32.4 mg; Mg(HCO3)2 = 14.6 mg and NaCl = 11.7 mg. Calculate temporary, permanent and total hardness of the water sample in ppm. (Atomic masses of Fe = 56; Ca = 40; Mg = 24 and Na = 23)
- If 5 teaspoonfuls of a 10% w/v povidone-iodine solution were diluted to 2 quarts with purified water, what would be the ratio strenght of the solution? (** Please show traditional method and not C1V1=C2V2 method**)Polar solutes were separated by normal-phase chromatography on bare silica, using methyl t-butyl ether and 2-propanol solvent. How would retention times be affected if eluent were changed from 40 vol% to 60 vol% 2-propanol?SHOW CLEAR SOLUTION: Using paper chromatography, a Chem 16 student tried to separate the components of an ink using 0.01% NaCl solution as mobile phase. His chromatogram is shown in figure 1. *rely on the picture* The student tried to identify whether component B can also be found in other pen brands so with the same mobile phase, he ran a paper chromatography experiment using brands X, Y, and Z. The results are shown in figure 2. A. Calculate the retention factors of components A, B, and C in figure 1.B. Arrange components A, B, and C in order of increasing polarity.C. From the results of his experiment, component B can also be found in which brand/s?
- Suppose that you try separating an unknown mixture by normal-phase chromatography with the solvent mixture 50% hexane-50% methyl t-butyl ether (which is more polar than hexane). The peaks are too close together and are eluted rapidly. Should you use a higher or lower percentage of hexane in the next run?A measurment for the Lambda-max for a colored solution is 671 nm with a very high Absorptivity. What does the color of the solution look like in the sunshine? and What does the color look like in a room where the only light source emits 671 nmMetal ions chelate polar groups of analyte molecules for longer retention time. Does this affect chromatogram of RPHPLC?