Problem 5. The ESI spectra of the protein alpha-lactalbumin is shown below. The molar mass of this protein is 14,178 Da. (a) Identify the peak that corresponds to a charged complex with overall charge +10. Show math to support your assignment. (b) Deduce the charge on the peak on the left and on the one right to the peak you identified in (a). 1289.90 100 1182.49 ndance

Problem 5. The ESI spectra of the protein alpha-lactalbumin is shown below. The molar mass of this protein is 14,178 Da. (a) Identify the peak that corresponds to a charged complex with overall charge +10. Show math to support your assignment. (b) Deduce the charge on the peak on the left and on the one right to the peak you identified in (a). 1289.90 100 1182.49 ndance

Biochemistry

9th Edition

ISBN:9781305961135

Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal

Publisher:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal

Chapter3: Amino Acids And Peptides

Section: Chapter Questions

Problem 10RE: MATHEMATICAL Calculate the isoelectric point of each of the following amino acids: glutamic acid,...

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Protein Isoelectric pH Molecular weight (kDa) Ovalbumin 4.6 45 Insulin 5.4 5.7 Fibrinogen 5.8 340 Y-globulin 6.6 160 Collagen Hemoglobin (monomer) 6.6 115 7.1 16 Myoglobin 7.0 16.7 From the given table, draw the appearance after visualization of a protein mixture containing the seven proteins in the table when subjected to SDS-PAGE.
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pH 6.2 Aspartate Glycine Histidine unknown Leucine Proline Lysine By looking at the electrophoretogram, and providing the answers in the box below, indicate: ) which of the known (!) amino acids are potentially at/ near their isoelectric point. Justify your answer by describing their relative migration compared to the origin and deduce their overall charge. i) Using one of these amino acids, describe how it meets the definition of being isoelectric by describing the protonation/ deprotonation state of all of the ionisable groups, and hence its overall charge. "