Put a primer on the top and bottom of bubble at origin. Identify 3’ ends. You can use a thicker line or different color. Primers are short (10-12 nucleotides). Draw two primers on lagging strand. Identify 3’ ends. Draw new DNA strands. Identify 3’ ends. New DNA is at least 10x longer than the primer. You don’t have to draw to scale but make it longer. Remember that the new DNA synthesis begins exactly at the 3’ end of the primer. Do not leave a gap (space) between 3’ end of primer and 5’ end of new DNA. Label leading and lagging strands. PRACTICE this with 5’ at top left and 3’ top left. If you can draw these two bubbles you can draw any of the 4 possible replication forks or any straight line section of DNA replication!

Human Heredity: Principles and Issues (MindTap Course List)
11th Edition
ISBN:9781305251052
Author:Michael Cummings
Publisher:Michael Cummings
Chapter15: Genomes And Genomics
Section: Chapter Questions
Problem 6QP: Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel,...
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  1. Put a primer on the top and bottom of bubble at origin. Identify 3’ ends. You can use a thicker line or different color. Primers are short (10-12 nucleotides).
  2. Draw two primers on lagging strand. Identify 3’ ends.
  3. Draw new DNA strands. Identify 3’ ends. New DNA is at least 10x longer than the primer. You don’t have to draw to scale but make it longer. Remember that the new DNA synthesis begins exactly at the 3’ end of the primer. Do not leave a gap (space) between 3’ end of primer and 5’ end of new DNA.
  4. Label leading and lagging strands.
  5. PRACTICE this with 5’ at top left and 3’ top left. If you can draw these two bubbles you can draw any of the 4 possible replication forks or any straight line section of DNA replication!
 
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