The functions of the loading dye are: (select all that apply) | to prevent sample degradation as gel generates heat to make sure sample sinks to bottom of sample wells so that you can visualize the sample as it runs through the gel so that you can see your bands under UV light|
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- Which of the following is/are source/s of error in performing a spectrophotometric method? Answer all that apply. Sample is turbid when the absorbance is read. The absorbances were read at maximum absorption. Standard solutions are prepared accurately. The cuvettes have fingerprints.SDS is required to unfold proteins during SDS-PAGE electrophoresis and it is included in both the loading and running buffer. If SDS is not included in the running buffer.... the proteins in your sample may partially renature thus affecting migration. the gel will not conduct current in the absence of SDS. there is no effect on the samples. the proteins in your sample will be graded.When loading samples onto a gel, why should expelling all the liquid in the tip be avoided? a)All the sample could be forced out from the well in which it was loaded. b)The extra liquid would end up in the buffer and this would distort the migration of the sample. c)The gel could tear and distort the migration of the sample. d)An air bubble could result which would distort the migration of the sample .
- In a polyacrylamide gel electrophoresis, the gel consists of specific gel components. name themWhich statement(s) describe(s) a microplate reader?A. It is spectrophotometerB. It can read the absorbance of one sample with adjustable path lengthC. It can handle multiple samples in parallelD. It will do all measurement jobs without our supervision a. A & B b. A, B & C c. A, B, C & D d. A e. A & CWhich spectrophotometer mode should you use to calibrate the instrument and which mode should you use to read your samples? Transmittance for both Absorbance for both Absorbance to calibrate and transmittance to read samples Transmittance to calibrate and absorbance to read samples Flip a coin to pick a mode each time.
- What are the mechanisms of samples separation work in Thin layer Chromatography? Please shortly write at your own words. Answer should be to the point (5-6 lines maximum).Which of the following statements is TRUE about the band of molecules labeled X ( close to where it says positive electrode). A) it contains DNA fragments that are shorter than the ones in any of the other bands. B) it contains fewer DNA molecules than any of the other bands from lane 3. C) it contains more negatively charged particles than any other band on the gel. D) it contains fewer genes than any of the other bands on the gel.A student performs a separation by TLC on a silica get plate that results in three spots. Ared spot is measured 23 mm away from the original sample spot, a blue spot is 42 mmaway, and a yellow spot is 49 mm away. The distance from the original spot to where thesolvent stopped is 55 mm.a) Calculate the Rf value for each component in the mixture. (Show your work and reportthe correct number of significant figures.) b) The silica gel plate is polar. Rank the three components in the mixture from lowest tohighest polarity. Explain your answer.
- Place the following steps for setting up an agarose gel in order : A. Let the gel solidify B. Turn on the current and run gel . C. Pour the molten agarose into the gel cast . D. Load ladder and DNA samples into the well . E. Assemble the gel cast including comb . F. Place lid on the gel running apparatus . G. Remove the comb and add running buffer. H. Orient the gel in the gel running apparatus . I. Add Sybr-safe to the molten agarose. J. Connect the leads to both the gel running apparatus and the power pack .Which of the following is NOT a function of the loading buffer used in SDS-PAGE? A) It contains a dye, letting you track the progression of electrophoresis samples B) It keeps the sample from floating away out of the gel well C) It measures the protein concentration of your samples D) It denatures proteins to turn them into linear chains of amino acidsYou will use a Calf Thymus DNA standard stock solution (1 mg/ml) to prepare a standard curve. Calculate volume of stock solution in ul, needed to prepare 10 ug/ml dilution (final volume 1.0 ml).