Reagents Sterile ddH₂0 10X PCR Buffer (E) mM MgCl₂ 10mM dNTP (1) mM Forward Primer 50 mM Reverse Primer Taq DNA polymerase DNA template Final Concentration (D) X 2mM (G) UM 0.5 mM 0.5 mM Volume (UL) Per 25ul Reaction (B) 2.5 1.0 0.5 0.25 (J) 0.2 2.0 Volume (ul) for (A)X reactions (C) 22.5 (F) (H) 2.25 2.25 (K) --

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Given the table, compute the volumes required for testing a panel of 6 samples plus the controls. 

 

 

Reagents
Sterile ddH₂0
10X PCR Buffer
(E) mM MgCl₂
10mM dNTP
(1) mM Forward Primer
50 mM Reverse Primer
Taq DNA polymerase
DNA template
Final
Concentration
(D) X
2mM
(G) UM
0.5 mM
0.5 mM
Volume (ul)
Per 25ul Reaction
(B)
2.5
1.0
0.5
0.25
(J)
0.2
2.0
Volume (ul) for
(A)X reactions
(C)
22.5
(F)
(H)
2.25
2.25
(K)
●●●
Transcribed Image Text:Reagents Sterile ddH₂0 10X PCR Buffer (E) mM MgCl₂ 10mM dNTP (1) mM Forward Primer 50 mM Reverse Primer Taq DNA polymerase DNA template Final Concentration (D) X 2mM (G) UM 0.5 mM 0.5 mM Volume (ul) Per 25ul Reaction (B) 2.5 1.0 0.5 0.25 (J) 0.2 2.0 Volume (ul) for (A)X reactions (C) 22.5 (F) (H) 2.25 2.25 (K) ●●●
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