Just Question 4 1Q2. How many different mRNAs could specify the amino acid sequence Met-Phe-Ser-Pro? List them.3. If the coding region of a gene is estimated to consist of 450 bp, how many amino acids would thecorresponding polypeptide chain contain? Estimate the molecular weight of the polypeptide chain(assume the average molecule weight of an amino acid is 110 Daltons).4. A single base addition and a single base deletion approximately 15 bases apart in the mRNA specifyingthe protein lysozyme from the bacteriophage T4 caused a change in the protein from its wild-typecomposition....Lys-Ser-Pro-Ser-Leu-Asn-Ala-Ala-Lys....to the double mutant form....Lys-Val-His-2SIWHis-Leu-Met-Ala-Lys..(a) From the mRNA codons in the genetic code (standard codon table), decipher the segment ofmRNA for both the original (wild-type) protein and the double mutant(b) Which base was added?(c) Which base was deleted?5. A eukaryotic cell produces a normal protein with following amino acid sequence: Met-Val-His-Lys-Arg-Thr-Leu-Val. After irradiation, a transformed (mutated) eukaryotic cell is produced that makes amutant protein from the same coding site on DNA: Met-Val-His-Lys-Glu-Pro. Describe the mutationthat occurred. As always, justify your answer.xAlt6. You are working with a newly discovered mutagen, and you wish to determine the base change that itintroduced into DNA. Thus far you have determined that the mutagen chemically alters a single base inn a way that its base-pairing properties are altered permanently. In order to determine thespecificity of the alteration, you examine the amino acid changes that place after mutagenesis. Asample of what you find is shown below:Original:Mutant:Original:Mutant:3EOriginal:Mutant:Arg-Ser-Leu-Trp-Lys-Thr-PheWhat is the base-change specificity of the mutagen? Present your evidence (justify).DGln-His-lle-Glu-LysGln-His-Met-Glu-LysсAla-Val-Asn-ArgAla-Val-Ser-ArgArg-Ser-Leu$RFV%5TG6BYHESCELLBIOS lectureppt ChO8.pptx&7NJ8MWednesday(91KO<WednesdayOPBenjamin Lunder 12 MD->Alt?I

As per the central dogma of molecular biology, the genetic information stored in the DNA is copied…

4. A single base addition and a single base deletion approximately 15 bases apart in the mRNA specifying the protein lysozyme from the bacteriophage T4 caused a change in the protein from its wild-type composition....Lys-Ser-Pro-Ser-Leu-Asn-Ala-Ala-Lys....to the double mutant form....Lys-Val-His- His-Leu-Met-Ala-Lys.... (a) From the mRNA codons in the genetic code (standard codon table), decipher the segment of mRNA for both the original (wild-type) protein and the double mutant (b) Which base was added? (c) Which base was deleted?

4. A single base addition and a single base deletion approximately 15 bases apart in the mRNA specifying the protein lysozyme from the bacteriophage T4 caused a change in the protein from its wild-type composition....Lys-Ser-Pro-Ser-Leu-Asn-Ala-Ala-Lys....to the double mutant form....Lys-Val-His- His-Leu-Met-Ala-Lys.... (a) From the mRNA codons in the genetic code (standard codon table), decipher the segment of mRNA for both the original (wild-type) protein and the double mutant (b) Which base was added? (c) Which base was deleted?

Biology 2e

2nd Edition

ISBN:9781947172517

Author:Matthew Douglas, Jung Choi, Mary Ann Clark

Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark

Chapter15: Genes And Proteins

Section: Chapter Questions

Problem 22CTQ: If mRNA is complementary to the DNA template strand and the DNA template strand is complementary to...

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Similar questions

If mRNA is complementary to the DNA template strand and the DNA template strand is complementary to the DNA nontemplate strand, then why are base sequences of mRNA and the DNA nontemplate strand not identical? Could they ever be?
1. Given the following three mRNA sequences, which 2 sequences code for the same protein?#1 AGU UUA GCA ACG AGA UCA#2 UCG CUA GCG ACC AGU UCA#3 AGC CUC GCC ACU CGU AGU 2. Look at the following sequence: THE FAT CAT ATE THE RAT. Delete the first H and regroup the letters in groups of three- write out the new groups of three. Does the sentence still make sense? What type of mutation is this an example of?
4A. In the human gene for the beta chain of haemoglobin (the oxygen-carrying protein in the red blood cells), the first 30 nucleotides in the amino-acid-coding region is represented by the sequence: 3'-TACCACGTGGACTGAGGACTCCTCTTCAGA-5'. What is the sequence of the partner strand? 4B. If the DNA duplex for the beta chain of haemoglobin above were transcribed from left to right, deduce the base sequence of the RNA in this coding region.
3b) In the real world, where "wobble" pairing is possible, what is the minimum number of tRNAs required to service all of the threonine codons? Write out the base sequences of the anticodons of those tRNAs (remember to label the 5' and 3' end of each anticodon sequence).
1. For the genotypes and conditions (lactose present or absent) shown in the following table predict whether functional enzymes, nonfunctional enzymes, or no enzymes are made. 2. Even though the LacZ,Y and A structural genes are transcribed as a single polycistronic mRNA, each gene contains the initiation and termination signals essential for translation. Predict what will happen when a cell growing in the presence of lactose contains a deletion of one nucleotide early in the Z gene and early in the A gene.
1. What is the nucleotide sequence of the mRNA transcribed from the template DNA strand: 3’-GCTACAAAAAGTCCATAATCGC-5’? Indicate the 5’ and 3’ ends. Follow the same format as the given sequence. 2. If the mRNA sequence you obtained in question 2 were to be translated, what would be the sequence of amino acids in the peptide? Use the 3-letter code of the amino acids. Separate the amino acids with a dash (-). NO NEED to indicate the N- and C-terminal amino acids. Assumption is that the first amino acid is the N-terminal. 3. If the mRNA sequence you obtained in question 2 were to be translated, what would be the sequence of amino acids in the peptide? Use the 3-letter code of the amino acids. Separate the amino acids with a dash (-). NO NEED to indicate the N- and C-terminal amino acids. The assumption is that the first amino acid is the N-terminal.
1.) if the B chain of human hemoglobin is 146 amino acids in length, calculate the minimum # of nucleotide base pairs needed to code for B-globin? 1a.) How many mRNA codons does this correspond to?
4. A mini mRNA has the sequence 5’-UUUGAAAUAUGAUUGAUAUUUAUAUAUGA-3. a) Using the genetic code, provide the amino acids specified by the mini mRNA. b) Label the two ends of the short peptide.
Shown below is a portion of a DNA sequence ( 31 base pairs long ) that encodes the last amino acids of a protein : The first three underlined base pairs indicate the frame and include the coding region . 123456789 A. Write the peptide sequence of the last 6 amino acids of the protein . Label both ends of the peptide . B. A insertion of one base pair causes the protein to decrease in length by 5 amino acids . With respect to the sequence given above , where does this insertion occur , and what base pair will you insert ? C. An change of one base pair leads to the protein to increase in length by one amino acid. With respect to the sequence given above , which base pair would you change ? How would you change this base pair for the protein to increase in length by one amino acid ?
How many binary sequences of length n contain at most five 1 digits? The genetic code specifies an amino acid through a sequence of three nucleotides. Each nucleotide can be of one of the four types T, A, C and G, beingrepetitions allowed. How many amino acids can be encoded in this way?And if there are n types. Compare
2a) Suppose you have a gene in which a single base substitution has created the nonsense mutation 5'TAA3' (which will be transcribed into 5'UAA3' in the mRNA - but recall that mutations are changes in the DNA sequence). Name all the amino acids that could have been coded for by the original, unmutated codon at that position in the gene.
1. A region of DNA in a particular cell synthesizes a segment of RNA that is 174 bases long and in the form of a large palindrome. The RNA is transported to the cytoplasm and folds into a hairpin loop of double stranded RNA. In the cytoplasm, the hairpin loop is recognized by a double-stranded RNA cutting enzyme (dicer) and is cut into 21 BP lengths. When these 21 BP double stranded segments are combined with protein, they function by: Answer choices destroying specific mRNAs priming the synthesis of DNA sequences adding DNA to the chromosome ends (telomeres) acting as decoys for RNA degrading enzymes thus protecting the mRNAs present splicing the introns out of messenger RNA 2. The following are genotypes of merozygotes of E. coli with various combinations of lac operon mutations. Determine the phenotype with respect to beta-galactosidase (z), permease (y), and trans-acetylase (a) of each combination as U = uninducible, I = inducible, and C = constitutive. Choose from…