RI-a Compound tested A B C DEG1 lod bos 2g 1 Mutanf 1 3. 4 101 + a. What is the order of compounds A to E in the pathway? b. At which point in the pathway is each mutant blocked? c. Would a heterokaryon composed of double mutants 1,3 and 2,4 grow on a minimal medium? Would 1,3 and 3,4? Would 1,2 and 2,4 and 1,4? | | |++ | + 1+ +
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
![15. Several mutants are isolated, all of which require com-
pound G for growth. The compounds (A to E) in the bio-
synthetic pathway to G are known, but their order in the
pathway is not known. Each compound is tested for its
ability to support the growth of each mutant (1 to 5). In
the following table, a plus sign indicates growth and a
minus sign indicates no growth.
olord bos og elu Compound tested
А В
B CDEG
Mutanf 1
a. What is the order of compounds A to E in the pathway?
b. At which point in the pathway is each mutant blocked?
c. Would a heterokaryon composed of double mutants
1,3 and 2,4 grow on a minimal medium? Would 1,3 and
3,4? Would 1,2 and 2,4 and 1,4?
+ + + +
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