Select one or more: O a. by the interaction of Rho and Rho binding sites in RNA. O b. by the interaction of ter site and Tus protein O c. by formation of a hairpin structure in RNA. O d. by formation of a hairpin structure of DNA at the end.
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- What is one function of TFIIH during transcription? a. Recruiting the TATA-box binding protein to the TATA-box. b. Terminating transcription via factor-dependent termination. c. Capping the 5' end of mRNA with 7-methylguanylate. d. Phosphorylating the CTD of RNA polymerase.Which of the following is/are a role for the poly-A tail? (Select all that apply.) a) Facilitates transport of the transcript out of the nucleus b) Confers stability to the mRNA c) Binds to RNA polymerase to initiate transcription d) Facilitates binding to DNAWhich of the following statements best describes the promoter of a protein-coding gene? A. The promoter is a protein that recruits RNA polymerase. B. The promoter is part of the RNA molecule itself. C. The promoter is a site found on RNA polymerase. D. The promoter contains the AUG start codon. E. The promoter is a nontranscribed region of a gene.
- How is transcription directly controlled in eukaryotic cells? a. through the use of phosphorylation b. by means of apoptosis c. using transcription factors and activators d. when chromatin is packed to keep genes turned on e. None of these are correct.Which of the following processes is required for the initiation of transcription in bacteria? Select one: a. binding of sigma to the promoter region b. formation of a peptide bond in the elongating polypeptide chain c. formation of a phosphodiester bond in the elongating RNA strand d. binding of DNA polymerase to the promoter regionMutation of which of these sequences would have an effect on translational initiation? A. 3’ UTR B. Polyadenylation signal C. 5’ UTR D. upstream enhancer E. TATA box
- Which of the following is not a reason that gene transcription is selective (occurs on specific segments of DNA)?Group of answer choices a) in eukaryotes, transcribed mRNA is processed to add a poly-A tail b) sigma factors direct RNA polymerase where to bind the DNA template c) promoters differ from gene to geneWhich of the following makes alternative RNA splicing possible? Group of answer choices A. Parts of a gene can function as exons or introns B. DNA methylation C. Parts of a gene can be either transcribed or not D. Histone Acetylation E. Parts of a gene can bind to RNA polymerase or notWhich of the following statements is/are TRUE for transcription? A. RNA polymerase uses one strand of DNA, the antisense strand, for RNA synthesis. B. The first ribonucleotide binds to the Pribnow region. C. RNA polymerase moves from 5’ → 3’, while the RNA polymerization is from 3’ → 5’. D. All four ribonucleoside monophosphates are required.
- Which of the following are steps of transcription? Select all that apply. A. RNA polymerase binds to a gene’s promoter. B. RNA polymerase moves over the gene and unzips the double helix to form a “transcription bubble.” C. RNA polymerase assembles a strand of mRNA complementary to the coding strand of DNA. D. RNA polymerase assembles a strand of mRNA complementary to the noncoding strand of DNA.Describe how it is possible to make a DNA copy of a gene and have that copy include an SspI site before the ATG and a SalI site after the stop codon, if the gene doesn’t already have these sites.Where will the promoter be relative to the start of transcription? Label the promoter. Where will the terminator be relative to the end of transcription? Label the terminator. What is the sequence of the mRNA produced from the template strand? Label the 5’ and 3’ ends.