Separate a mixture of Vitamin D and Vitamin B2 (riboflavin) by paper chromatography. The stationary phase is methanol (CH;OH) and the mobile phase is pentane (CSH12). Place the vitamins in order of elution. Explain why using IMF. (Circle polar sites on each molecule and label all 4 molecules as polar or non-polar.) Vitamin B, CH, H,C CH OH Vitamin D Он CH,OH
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- Based on this. What was the percentage error in the percentage of water in the sample, compared to pure MgSO4•7H2O?A spot of crude plant extract was placed on a glass plate coated with C18 silica gel. The chromatogram was developed using an appropriate solvent. The result is shown below. Which of the following spot is the most soluble in water? Choices: Spot C Spot M Spot E Spot HWhat is the function of a desiccant in a set-up of volatilization gravimetry? To remove nitrogen from the sample To remove sulfuric acid from the sample To remove water vapor from the sample To remove carbon dioxide from the sample
- The solubility of compound B is 59 g per 100 mL in boiling methanol and 30 g per 100 g in cold methanol, while its solubility in water is 7.2 g per 100 mL at 95oC and 0.22 g per 100 mL at 20oC. What solvent would be better for recrystallization of the compound? ExplainImagine you are attempting to separate these three dipeptides from each other using anion exchange chromatography, and you use a protein assay to identify at what salt concentration each of them is eluted from the column. A graph showing protein concentration as a function of salt concentration is shown below the dipeptides. Which of the three dipeptides is represented by peak #3? (NOTE: the dipeptides are shown in their non-ionized forms. You must assume that they are in aqueous solution at physiological pH, ~7.2) a) Dipeptide A b) Dipeptide B c) Dipeptide CEnumerate at least two important things to do to maximize yield when using vacuum filtration in the synthesis of aspirin.
- A spot of crude plant extract was placed on a glass plate coated with C18 silica gel. The chromatogram was developed using an appropriate solvent. The result is shown below. Which of the following spot is the most soluble in water?Calculate the vapor pressure of propanol (b.p. 97.0 ∘C97.0 ∘C) at the gas chromatography column temperature of 95.0 ∘C95.0 ∘C using the form of the Clausius–Clapeyron equation shown ln(?1?2)=−(Δ?vap?)(1?1−1?2)ln(P1P2)=−(ΔHvapR)(1T1−1T2) where ?R is the ideal gas constant, Δ?vapΔHvap is the enthalpy of vaporization, ?1T1 and ?2T2 are two different temperatures, and ?1P1 and ?2P2 are the vapor pressures at the respective temperatures. The enthalpy of vaporization can be estimated using Trouton's rule, Δ?∘vap=(88 J·mol−1⋅K−1)×?bp.ΔHvap∘=(88 J·mol−1⋅K−1)×Tbp. ?=P= TorrTorr Calculate the vapor pressure of pentane (b.p. 36.0 ∘C36.0 ∘C) at the same column temperature. ?=P= TorrTorr For compounds of the same chemical class, what is the relationship between vapor pressure of a compound and retention in gas chromatography? As the vapor pressure decreases, retention time decreases. As the vapor pressure increases, retention time increases. As…4) If the classification of the chromatography run is a reverse-phase chromatography, discuss the relative polarities of caffeine, aspartame and benzoate.5) Given the data of the diet sodas, which one would you recommend to be the healthiest? (You may need to research the functions of caffeine, aspartame and benzoate. Please add references if necessary.)
- 15 g of unknown organic sample was dissolve in 575 mL of Dicloromethane (DCM). The boiling point of benzene was increased by 3.40oC. Determine the molecular weight of the unknown sample? Kb of DCM = 2.42oC/m Bb of benzene = 39.6 oC density of benzene = 1.33 g/mL at 25 °Cin a chromatographic analysis of water from a drinking fountain gives area of 1.56*10^4, 5.12*10^4, 1.49*10^4 and 1.76*10^4 for CH3CL3, CHCL2BR, CHCLBR2 and CHBR3 respectively. determine the percentage concentration of each of the triichoromethane in the sampleConsider the following statements as they apply to gas chromatography. Decide if each is true or false. 1. ___ Temperature programming is necessary only for samples containing compounds with a narrow range of boiling points. 2. ___ GC carrier gases are generally inert gases like helium and nitrogen. 3. ___ The injection port temperature should be set at the average boiling point of all analytes. 4. ___ An FID is a mass-sensitive detector, not a concentration-sensitive device. 5. ___ An air peak is commonly used to determine the dead time when using an FID detector.