Shown below is a theoretical titration curve of histidine (amino acid). Use the provided titration curve in answering the questions asked. Hd 14 12 10 8 6 4 2 0 0 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 Volume of Titrant / mL Histidine 0.10 mol/L - titrant = NaOH 0.1 mol/L 1. Which of the following forms of Histidine is/are amphoteric? (More than one answer may apply) a. Hist b. His c. His2+ d. His 5. What is the charge of the most protonated form of histidine? a. 0 b. +1 C. +2 d. +3 e. +4

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
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Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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Shown below is a theoretical titration curve of histidine (amino acid). Use the
provided titration curve in answering the questions asked.
Hd
14
12
10
8
6
4
2
0
0 2
4
6
8
10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40
Volume of Titrant / mL
Histidine 0.10 mol/L - titrant = NaOH 0.1 mol/L
1. Which of the following forms of
Histidine is/are amphoteric? (More
than one answer may apply)
a. Hist
b. His
c. His2+
d. Hiso
2. According to the titration curve
above, what is the isoelectric point of
histidine? Make sure to place your
answer up until the first decimal
point.
3. Which form of histidine is the most
abundant at pH 10.0?
a. Hist
b. His
c. His2+
d. His
c. His2+
d. Hisº
4. Which form of histidine is the most
abundant at pH 7.0?
a. Hist
b. His
5. What is the charge of the most
protonated form of histidine?
a. 0
b. +1
C. +2
d. +3
e. +4
6. In the titration curve of the amino
acid above, how many buffering
regions are present?
7. True or False: All amino acids have
zwitterions
Transcribed Image Text:Shown below is a theoretical titration curve of histidine (amino acid). Use the provided titration curve in answering the questions asked. Hd 14 12 10 8 6 4 2 0 0 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 Volume of Titrant / mL Histidine 0.10 mol/L - titrant = NaOH 0.1 mol/L 1. Which of the following forms of Histidine is/are amphoteric? (More than one answer may apply) a. Hist b. His c. His2+ d. Hiso 2. According to the titration curve above, what is the isoelectric point of histidine? Make sure to place your answer up until the first decimal point. 3. Which form of histidine is the most abundant at pH 10.0? a. Hist b. His c. His2+ d. His c. His2+ d. Hisº 4. Which form of histidine is the most abundant at pH 7.0? a. Hist b. His 5. What is the charge of the most protonated form of histidine? a. 0 b. +1 C. +2 d. +3 e. +4 6. In the titration curve of the amino acid above, how many buffering regions are present? 7. True or False: All amino acids have zwitterions
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