Site specific recombination using Cre-Lox system shown below will result in JoxP foxP faaP Fed JoP
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- An Hfr strain that is hisE+ and pheA+ was mixed with a strain thatis hisE− and pheA−. The conjugation was interrupted and the percentageof recombinants for each gene was determined by streakingon a medium that lacked either histidine or phenylalanine. Thefollowing results were obtained:It was found that hisE is 4 minutes away from pabB. The gene pheA was shown to be 17 minutes from pabB. Draw a genetic map describing the locations of all three genes.By conducting conjugation experiments between Hfr and recipientstrains, Wollman and Jacob mapped the order of many bacterialgenes. Throughout the course of their studies, they identified severaldifferent Hfr strains in which the F-factor DNA had been integratedat different places along the bacterial chromosome. A sample of theirexperimental results is shown in the table:Draw a map that shows the order of genes and the locations ofthe origins of transfer among these different Hfr strains?In the Holliday model for homologous recombination shown, the resolution steps can produce recombinant or nonrecombinantchromosomes. Explain how this can occur.
- A particular Hfr strain normally transmits the pro+marker as the last one in conjugation. In a cross of thisstrain with an F− strain, some pro+ recombinants are recovered early in the mating process. When these pro+cells are mixed with F−cells, the majority of the F− cellsare converted into pro+ cells that also carry the F factor.Explain these results.True or False. Chromatin remodeling is required for both homologous recombination and non-homologous end joining.An Hfr strain that is hisE + and pheA + was conjugated to a strain that is hisE − and pheA −. The conjugation was interrupted at different times, and the percentage of recombinants for each gene was determined by streaking on media that lacked either histidine or phenylalanine. The following results were obtained: A. Determine the map distance (in minutes) between these twogenes.B. In a previous experiment, it was found that hisE is 4 minutesaway from pabB and that PheA is 17 minutes from pabB. Drawa genetic map showing the locations of all three genes.
- Define the following terms:a. nonreplicative transpositionb. replicative transpositionc. composite transposond. retrotransposone. insertional elementAnalyzing Cloned Sequences A base change (A to T) is the mutational event that created the mutant sickle cell anemia allele of beta globin. This mutation destroys an MstII restriction site normally present in the beta globin gene. This difference between the normal allele and the mutant allele can be detected with Southern blotting. Using a labeled beta globin gene as a probe, what differences would you expect to see for a Southern blot of the normal beta globin gene and the mutant sickle cell gene?In gene targeting, homologous recombination between thebacterial chromosome and a linear _______- construct synthesized in vitro can generate a null mutation in any gene.
- V/D/J recombination frequently yields a gene whose mRNA cannot be successfully translated into an immunoglobulin chain. What aspect of somatic recombination is likely to produce nonproductive gene rearrangement?By conducting conjugation experiments between Hfr and recipientstrains, Wollman and Jacob mapped the order of many bacterialgenes. Throughout the course of their studies, they identified severaldifferent Hfr strains in which the F-factor DNA had been integratedat different places along the bacterial chromosome. A sample of theirexperimental results is shown in the following table:Analyze data. Compare and contrast. Make a drawing.During recombination by the double strand break mechanism what is the structure of the intermediate with holiday functions pair to branch migration?Draw the structure taking three dominant alleles (ABC) on the parental duplex donoted by thin back lines and the parental duplex denoted by thick lines has recessive alleles (abc) show the difference between the DNA chain from the parental duplex and discuss the molecular mechanisms involved
