3. Solve the sequence of an oligopeptide 7 residues long which gave:Asp Leu Lys Met Phe TyrThe following facts were observed:a. Trypsin treatment had no apparent effectb. The PTH derivative from Edman degradation was c. Brief chymotrypsin treatment yielded several products including but not limited to a dipeptide and a tetrapeptide. The amino acid composition of the tetrapeptide was Leu, Lys, and Met.d. Cyanogen bromide treatment yielded a dipeptide, a tetrapeptide, and a free Lys. ReagentCleavage PointsTrypsinLys, Arg (C)Submaxillary proteaseArg (C)ChymotrypsinPhe, Trp, Tyr (C)2/4Staphylococcus aureusAsp, Glu (C)Asp-N-proteaseAsp, Glu (N)PepsinLeu, Phe, Trp, Tyr (N)Endoproteinase Lys CLys (C)Cyanogen bromideMet (C)Carboxypeptidase AWorks on all residues (C) except Pro, Arg, Lys,Asp, and GluCarboxypeptidase BArg, Lys (C) 3. Solve the sequence of an oligopeptide 7 residues long which gave:Asp Leu Lys Met Phe TyrThe following facts were observed:a. Trypsin treatment had no apparent effectb. The PTH derivative from Edman degradation was-Ċ-CH 2H.c. Brief chymotrypsin treatment yielded several products including but not limited to a dipeptideand a tetrapeptide. The amino acid composition of the tetrapeptide was Leu, Lys, and Met.d. Cyanogen bromide treatment yielded a dipeptide, a tetrapeptide, and a free Lys.

Oligopeptide contains 2-20 amino acids. The amino acids are joined by the peptide bond.

Solve the sequence of an oligopeptide 7 residues long which gave: Asp Leu Lys Met Phe Tyr The following facts were observed: a. Trypsin treatment had no apparent effect b. The PTH derivative from Edman degradation was c. Brief chymotrypsin treatment yielded several products including but not limited to a dipeptide and a tetrapeptide. The amino acid composition of the tetrapeptide was Leu, Lys, and Met. d. Cyanogen bromide treatment yielded a dipeptide, a tetrapeptide, and a free Lys.

Solve the sequence of an oligopeptide 7 residues long which gave: Asp Leu Lys Met Phe Tyr The following facts were observed: a. Trypsin treatment had no apparent effect b. The PTH derivative from Edman degradation was c. Brief chymotrypsin treatment yielded several products including but not limited to a dipeptide and a tetrapeptide. The amino acid composition of the tetrapeptide was Leu, Lys, and Met. d. Cyanogen bromide treatment yielded a dipeptide, a tetrapeptide, and a free Lys.

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter15: From Dna To Protein

Section: Chapter Questions

Problem 4TYK

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3. Solve the sequence of an oligopeptide 7 residues long which gave: Asp Leu Lys Met Phe Tyr The following facts were observed: a. Trypsin treatment had no apparent effect b. The PTH derivative from Edman degradation was c. Brief chymotrypsin treatment yielded several products including but not limited to a dipeptide and a tetrapeptide. The amino acid composition of the tetrapeptide was Leu, Lys, and Met. d. Cyanogen bromide treatment yielded a dipeptide, a tetrapeptide, and a free Lys. Instructions Make use of the table below to determine the sequence of the mystery protein.
2. A group of peptides that influence nerve transmission in certain parts of the brain have been isolated from normal brain tissue. These peptides are known as opioids. Using the information below, determine the amino acid sequence of this particular opioid: ● Complete hydrolysis by HCl at 100° C followed by amino acid hydrolysis analysis indicated the presence of Gly, Leu, Phe, and Tyr, in a 2:1:1:1 ratio. ● Treatment of the peptide with FNDB followed by complete hydrolysis indicated the presence of 2,2-dinitrophenyl derivative of tyrosine. No free tyrosine could be found. ● Complete digestion of the peptide with chymotrypsin yielded free tyrosine and leucine, plus a tripeptide containing Phe and Gly in a 1:2 ratio. Instructions Make use of the table below to determine the sequence of the mystery protein:
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