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- What does the %RSD, listed in the spectral data, as well as the correlation coefficient of your standard curve, tell you about the quality of the data and integrity of the results? my standard % RSD is 36.4%, I had to calculate manually from the 5 standard solutions I did have on the spectra. The R squared value from the standard curve is 0.7282I have set up my spreadsheet using this sample data and reproduced the result with a pKa of 4.64. true or falseA second spectrophotometric method for the quantitative analysis of Pb2+ in blood yields an Ssamp of 0.712 for a 5.00 mL sample of blood. After spiking the blood sample with 5.00 μL of a 1560 ppb Pb2+ external standard, an Sspike of 1.546 is measured. What is the concentration of Pb2+ in the original sample of blood?
- a sample of butyl benzoate is prepared to use as an internal standard. The results of a preliminary run, which used a solution known to contain 1.53 mg/mL of methyl benzoate (peak A) and1.68 mg/mL of butyl benzoate (peak B), are shown. The area of peak A is determined to be 352 and the area of peak B is determined to be 416 measured in arbitrary units by the computer. To measure the sample, 1.00 mL of a standard sample of butyl benzoate containing 2.19 mg/mL is mixed with 1.00 mL of the plant stream material. Analysis of the mixture gave a peak area of 449 for peak A and 411 for peak B. What is the concentration of methyl benzoate in the plant stream? concentration of methyl benzoate = ____________________ mg/mL1 mL was taken from the sample filtrate and mixed with 13 mL pure water and 4 mL sulfomolybdic acid and 2 mL dilute SnCI2 solution by adding it, and after waiting for 15 minutes, the absorbance of the resulting solutions against pure water was read at 520 nm. if the function of the calibration graph obtained with standard phosphorus solutions of 0.5-2.5 mg/mL is y= 0.245x + 0.107 and the absorbance of the serum sample is 0.342, how many grams of phosphorus is the amount in the sample?An analyst determines the Cl^- content of rain water with ion chromatography. The data for the calibration line in the range 5 to 50 milli equivalents per liter (mEq/L) is shown in the table below. Additionally, a blank sample was analysed 5 times and the following results obtained: 1.1, 0.5, 0.1, 0.9 and 1.2 ppm Ct Calculate the detection limit (DL) for the method with a 98.3% confidence level. Use ALL data and show equations
- Before determining the amount of Na2CO3 in an unknown sample, a student decides to check her procedure by analyzing a sample known to contain 98.76% w/w Na2CO3. Five replicate determinations of the %w/w Na2CO3 in the standard were made with the following results 98.71% 98.59% 98.62% 98.44% 98.58% Is the mean for these five trials significantly different from the accepted value at the 95% confidence level (α = 0.05)A new method is proposed to simultaneously detectand separatefour foodadditives by reversed-phase liquid chromatography. The data were obtained using theliquid chromatographic column with a flow rate of 0.5 mL per min and with the followingparameters: Pore size 100 A Particle Size 3.5 Internal diameter 4.6 mm Length 150 mm The food additives found in the sample resulted in the following data: ( see image for table) a.Calculate the number of plates from each peak. b.Calculate the plate height for the column. c.Retention factor for each peak.4.Consider the peaks for pentafluorobenzene and benzene in the gas chromatogram shown here. The elution time for unretained solute is 1.06 min. The open tubular column is 30.0 m in length and 0.530 mm in diameter, with a layer of stationary phase 3.0 μm thick on the inner wall. a) Measruing the width, w, at the baseline on the chromatogram, find the number of plates for these two compounds b) Use your answer to (a) to find the resolution between the two peaks c) Using the number of plates N=sqrtN1*N2 with the values from (a) calcuate what the resolution should be and compare your answer with the measured resolution in b
- 110.89 mg of an impure sample of the drug was diluted in 100 ml and injected in a HPLC, the AUC of the major peak in the chromatogram integrated 1294 units. A standard solution containing 1.01 mg/ml of this drug was then injected in the same HPLC and the resulting peak integrated 2228 units. What is the %w/w of purity for this sample?(One decimal place, no units)A spectrophotometric method for the quantitative determination of the concentration ofPb2+ in blood yields an Ssamp of 0.133 for a 1 mL sample of blood that has been diluted to 6 mL. A second sample is spiked with 1 µL of a 1467 ppb Pb2+ standard and diluted to 6 mL, yielding an Sspike of 0.491. Determine the concentration of Pb2+ in the original sample of blood.You prepare the following MeOH standards from a 2000 ppm stock MeOH solution:Calibration Blank, 50 ppm, 100 ppm, 150 ppm and 400 ppm and analyze them using the GC-FID. You received the following data: Concentration of MeOH(ppm) Response (area) of MeOH peak (pA) 0 0 50 150 100 320 150 440 400 1100 Using the data above, make a properly documented graph of "Response (area)”versus "Concentration of MeOH (ppm)" and determine the linear workingrange (in units of concentration) of MeOH. This graph is to be done usingExcel. Be sure to provide a trendline, formula & R2 value).