Test for Phosphate 1. Mix 1 mL of hydrolysate and 3 mL of NH4OH solution. 2. Add 10% HNO3 solution until acidic to litmus. 3. Add 2 mL of 0.2 M ammonium molybdate solution. 4. Warm in a boiling water bath for 2 minutes. Observe for the formation of yellow precipitate. *What is the chemical name and formula of the precipitate? _________________________________
*Test for Phosphate
1. Mix 1 mL of hydrolysate and 3 mL of NH4OH solution. 2. Add 10% HNO3 solution until acidic to litmus. 3. Add 2 mL of 0.2 M ammonium molybdate solution. 4. Warm in a boiling water bath for 2 minutes. Observe for the formation of yellow precipitate.
*What is the chemical name and formula of the precipitate? _________________________________
1. Suppose Biuret test is conducted to a solution of RNA. Will it give a positive result or not? Explain your answer. ____________________________________________________________________ ____________________________________________________________________
2. Why is Molisch’s test used for the determination of presence of pentose in the hydrolysate? What other test could be used for this? ____________________________________________________________________ ____________________________________________________________________
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