that absorbs at the wavelength of analysis will not obey Beer Lambert’s law. In uv-vis spectroscopy, the higher the absorbance the higher the % transmittance. In uv-vis spectroscopy, shining light to any substance would result in absorbance of the light by the substance regardless of energy of the incident light. Only samples with inherent uv-vis absorbing material can be analyzed by a uv-vis spectrometer.

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Spectroanalytical Methods

Spectroanalytical methods measure the wavelength and intensity of radiations that are either emitted or absorbed by a molecular or atomic species. These methods are useful for evaluating the electronic or atomic arrangement of the molecules of a given analyte.