The diagram to the right represents one technique used in biotechnology. The organic compound used to cut the bacterial DNA so that the human DNA could be inserted a(n)

Q: Next generation sequencing using Illumina platforms identifies individual bases in a DNA fragment…

A: The following steps are involved in next generation sequencing: 1) First the DNA sequencing…

Q: What do you think are the crucial steps in Polymerase Chain Reaction technique? Enumerate the…

A: PCR (polymerase chain reaction) the reaction mixture includes the DNA extract (template DNA), Taq…

Q: Gel electrophoresis can be used for all of the following purposes except. (A) Determining the…

A: Gel electrophoresis is the technique of separating macromolecules like DNA, RNA and protein with the…

Q: contrast to white rice. Using recmbinant technology, golden rice is created by added two new…

A: There are six steps involved in rDNA technology. These are – isolating genetic material, restriction…

Q: 5.Statement 1: Restriction enzymes are sequence-specific, which means that they only recognize a…

A: Restriction enzymes also known as restriction endonucleases are the DNA cutting enzymes that are…

Q: ing the gel picture of the DNA ladder, show where your digestion of the paper cutting model showing…

A: DNA is a double stranded biomolecule. The DNA is usually digested by endonucleases and exonucleases…

Q: DNA fingerprinting uses a process called gel electrophoresis to separate the fragments of DNA. Once…

A: Gel electrophoresis is considered a molecular technique to separate the DNA fragments as per their…

Q: The DNA sequence below is used by the primase to synthesize a primer. What is the sequence of the…

A: The primer of this sequence will be complementary to it. The complementary bases are as follows: A…

Q: Match the activity below with the correct enzyme. (You won't use all the enzymes listed.) RNA acts…

A: DNA helicase is used during the DNA replication process where it binds to the double-stranded DNA…

Q: The original DNA template starts with T following the primer sequence if the smallest fragment…

A: Dideoxy nucleotides are similar to regular, or deoxy, nucleotides, but they lack a  3’ OH group.…

Q: 4. The image below is based on the Meselson Stahl experiment where bacteria are grown in 15N and…

A: The Meselson-Stahl experiment showed that DNA replication is a semiconservative process. In this…

Q: RECOMBINANT DNA BRIEFLY, DESCRIBE RECOMBINANT DNA AND GIVE ONE CONCRETE EXAMPLE. EVALUATE THE…

A: Through recombinant DNA technology, we can clone our desired gene into a plasmid to get the desired…

Q: Gel electrophoresis can be used to separate DNA on the basis of:   A. the probe used.   B. size.…

A: Electrophoresis is a laboratory technique used to separate DNA, RNA or protein molecules. Agarose…

Q: 1. Why do we need to check the isolated DNA for its quantity and quality? 2. The purity of DNA…

A: Deoxyribonucleic acid DNA isolation is referred to the extraction process of DNA from different…

Q: 1. You have the plasmid pUC18/19, which is a circular plasmid that consists of 2686 bp. What would…

A: As per our guidelines, we are supposed to answer only one question. Kindly repost the other question…

Q: TRUE OR FALSE: a) Okazaki fragments are short DNA pieces that explain how the DNA polymerase can…

A: DNA replication process is semi conservative. The semi conservative replication of DNA refers to the…

Q: 4. Animation 12.4: This table shows results from a genetic test conducted on DNA samples from two…

A: Restriction enzymes are the enzymes capable of cutting double-helical segments that contain a…

Q: Would you choose an exonuclease while producing a recombinant DNA molecule?

A: Exonuclease eliminates nucleotides from the ends of the DNA and thus it can't help in secluding…

Q: 5. Below is an image of DNA sequenced using the Sanger sequencing method, where different…

A: Gel electrophoresis is a molecular technique that aims to separate the DNA, RNA, and protein…

Q: What are sticky ends? State their significance in recombination DNA technology.

A: Recombinant DNA Technology is a method for creating artificial DNA by combining genetic resources…

Q: You have a DNA sequence of 1400 bp. To characterize it, you decide to make a restriction map.…

A: DNA is a nucleotide polymer present in living beings.

Q: The same restriction endonuclease must be used to excise the foreign DNA and bacterial DNA. Select…

A: DNA or deoxyribonucleic acid is a double-stranded molecule, strands of which are coiled around one…

Q: DNA Polymerase Holoenzyme Il is represented below. It consists of several domains with distinct…

A: At the replication fork, DNA polymerase III holoenzyme (Pol III HE) works with the helicase and…

Q: From where do we get primers for sequencing DNA? A) they are synthesized by reverse transcriptase…

A: Primer is a short piece of ribonucleic acid sequence that provides a starting point for DNA…

Q: Because DNA can only be synthesized in the 5' to 3' direction...   A. The template DNA must be…

A: DNA stands for deoxyribonucleic acid. It is the genetic material of the organisms that transfer from…

Q: 2. Your Genetics professor gives you and your classmate's information on a restriction digest. The…

A: The advantages of biotechnology for our society can be in drug manufacturing at a low cost using…

Q: SANGER SEQUENCING NEXT GENERATION SEQUENCING  WRITE A PRECISE AND ACCURATE DIFFERENTIAL REPORT ON…

A: sanger sequencing and next-gen sequencing is that the sanger method only sequences a single DNA…

Q: Your colleague made the table below comparing DNA Polymerase to RNA Polymerases. Which row is…

A: Transcription is the molecular process which involves the DNA sequences that are transcribed as…

Q: _1. Bacterial proteins that have the ability to cut both strands of the DNA molecule at certain…

A: DNA and RNA are the two main forms of nucleic acids. Nucleotides, which have a five-carbon sugar…

Q: The Southern blot procedure was developed so that: DNA fragments that have been subjected to…

A: Southern blotting is the technique for the detection of specific DNA fragments. The detailed…

Q: The diagram shows an autoradiograph of a DNA sequencing gel. What is the 5' to 3' sequence of the…

A: Gel electrophoresis is a technique which is used to separate the molecules based on the size of the…

Q: double-stranded length of DNA is exposed to a restriction enzyme.  The enzyme finds 3 recognition…

A: Restriction enzymes Restriction enzymes are the specific enzymes that cleave the DNA in very…

Q: Recombinant DNA techniques that require gel electrophoresis

A: Following are the applications of gel electrophoresis in DNA recombinant technology.

Q: The following statements are true about common gene cloning procedures except: -DNA plasmids can…

A: Biotechnology is a branch of biology, including the use of living organisms to produce products.…

Q: 9. Five DNA samples are shown along with their recognition sites where a particular restriction…

A: Gel electrophoresis Gel electrophoresis is a molecular technique that involves the separation of DNA…

Q: 1. The following gel was produced in manual DNA sequencing. What would the unknown DNA template be…

A: The method of determining the nucleic acid sequence – the order of nucleotides in DNA – is known as…

Q: PCR is a powerful technique to screen and amplify segments of DNA for use in recombinant protein…

A: The polymerase chain reaction is abbreviated as PCR. It is a molecular biology technique for…

Q: 5. A scientist was studying a fragment of eukaryotic DNA that she suspected was involved in a…

A: A restriction endonuclease or restriction enzyme is a bacterial enzyme that cuts dsDNA into…

Q: Which of the following is the correct order in extracting the DNA?   I. Washing of DNA II. Tissue…

A: DNA act as genetic material in most of organism . It is two stranded , ladder like structure . DNA…

Q: Please answer both parts, a. The enzyme that catalyzes the joining of fragments to form recombinant…

A: The recombinant DNA technology is a  branch of biology that deals with different techniques that…

Q: What is the purpose of restriction enzyme? 2. What is the purpose of the probe in DNA finger…

A: Since you have posted a question with multi- sub parts , we will solve first three sub-parts for…

Q: Restriction enzymes in bacterial cytoplasm cut injected bacteriophage DNA wherever certain sequences…

A: Enzymes are the protein which acts as catalyst in the chemical reactions. Enzymes neither takes part…

Q: a) What are vectors? Describe extensively the roles vectors play in genetic engineering? Write short…

A: Note: Solving answer only for part A as per the guidelines, repost remaining part, please. The…

Q: Which of the following statements about gel electrophoresis is false? Choose all that apply.…

A: Gel electrophoresis is a technique used in the separation of DNA fragments .

Q: Restriction enzymes are used in laboratory manipulations to cut DNA; many of these enzymes can be…

A: Restriction enzyme - Restriction enzyme are also called as Restriction endonuclease. restriction…

Q: Make simple steps to extract isolating DNA from strawberries and yeast.

A: Answer

Q: PCR is essential for Select one: A. allowing restriction enzymes to cut DNA. B. making many copies…

A: Polymerase chain reaction (PCR) is a typical research facility method used to make many duplicates…

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.