The following are steps in DNA cloning and isolation of a specific fruit Ripening Gene from avocado. From ONLY the following steps below, which step comes FOURTH?? Colonies containing a specific gene are identified by colony hybridization and chosen for further analysis Avocado cells are lysed and DNA is extracted Ampicillin-resistant, LacZ- recombinant cells are selected for further analysis O Ligated recombinant vectors are transformed into E. coli cells and selected for ampicillin resistance Avocado DNA and plasmid vector DNA are both cut with the restriction enzyme, Bam-H1

Biology Today and Tomorrow without Physiology (MindTap Course List)
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Chapter10: Biotechnology
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The following are steps in DNA cloning and isolation of a specific fruit Ripening Gene from avocado.
From ONLY the following steps below, which step comes FOURTH??
O Colonies containing a specific gene are identified by colony hybridization and chosen for further analysis
O Avocado cells are lysed and DNA is extracted
Ampicillin-resistant, LacZ- recombinant cells are selected for further analysis
Ligated recombinant vectors are transformed into E. coli cells and selected for ampicillin resistance
Avocado DNA and plasmid vector DNA are both cut with the restriction enzyme,
Bam-H1
Transcribed Image Text:The following are steps in DNA cloning and isolation of a specific fruit Ripening Gene from avocado. From ONLY the following steps below, which step comes FOURTH?? O Colonies containing a specific gene are identified by colony hybridization and chosen for further analysis O Avocado cells are lysed and DNA is extracted Ampicillin-resistant, LacZ- recombinant cells are selected for further analysis Ligated recombinant vectors are transformed into E. coli cells and selected for ampicillin resistance Avocado DNA and plasmid vector DNA are both cut with the restriction enzyme, Bam-H1
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